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Food Analytical Methods

, Volume 12, Issue 11, pp 2535–2541 | Cite as

Colorimetric Detection of Horse Meat Based on Loop-Mediated Isothermal Amplification (LAMP)

  • Jiayu Wang
  • Yuping Wan
  • Gangyi Chen
  • Hengxing Liang
  • Sheng Ding
  • Ke Shang
  • Mei Li
  • Juan Dong
  • Feng Du
  • Xin Cui
  • Zhuo TangEmail author
Article
  • 46 Downloads

Abstract

Meat authentication and meat adulteration have become a growing global concern leading to consumers’ demand for accurate meat identification methods. Since existing methods for meat identification are cumbersome and require specialized equipment and/or training, we developed a specific colorimetric detection method that can rapidly identify horse meat as few as 0.1% contamination in xenogeneic meat sources within 1 h with no false-positive signals observed. Specificity analysis revealed no cross-reactivity with 14 other animal species. Therefore, a rapid on-site survey based on our method can be set up to identify undeclared meat in the food supply chain and contributes to an approach on food analysis by meeting the demands for quick and easy-to-perform analytical methods.

Keywords

Horse meat LAMP Colorimetric On-site detection 

Abbreviations

FSAI

Food Safety Authority of Ireland

PBZ

phenylbutazone

ELISA

enzyme-linked immunosorbent

mtDNA

mitochondrial DNA

real-time PCR

real-time polymerase chain reaction

PCR-RFLP

PCR-based restriction fragment length polymorphism

LAMP

loop-mediated isothermal amplification

COI gene

cytochrome c oxidase subunit I gene

HNB

hydroxy naphthol blue

Notes

Funding Information

This work was supported by the National Natural Science Foundation of China [grant numbers 21572222, 21708037, 21877108], the Innovative Team of Sichuan Province [grant number 2017TD0021], Chengdu Municipal Bureau of Science and Technology [grant number 2015-HM02-00099-SF, 2016-HM01-00371-SF], and China Postdoctoral Science Foundation [grant number 2017M612998].

Compliance with Ethical Standards

Conflict of Interest

Jiayu Wang declares that she has no conflict of interest. Yuping Wan declares that she has no conflict of interest. Gangyi Chen declares that he has no conflict of interest. Hengxing Liang declares that he has no conflict of interest. Sheng Ding declares that he has no conflict of interest. Ke Shang declares that he has no conflict of interest. Mei Li declares that she has no conflict of interest. Juan Dong declares that she has no conflict of interest. Feng Du declares that she has no conflict of interest. Xin Cui declares that she has no conflict of interest. Zhuo Tang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1590_MOESM1_ESM.docx (2.9 mb)
ESM 1 (DOCX 3014 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jiayu Wang
    • 1
    • 2
  • Yuping Wan
    • 3
  • Gangyi Chen
    • 1
  • Hengxing Liang
    • 3
  • Sheng Ding
    • 1
  • Ke Shang
    • 3
  • Mei Li
    • 1
  • Juan Dong
    • 1
  • Feng Du
    • 1
  • Xin Cui
    • 1
  • Zhuo Tang
    • 1
    • 2
    Email author
  1. 1.Natural Products Research Center, Chengdu Institute of BiologyChinese Academy of SciencesChengduPeople’s Republic of China
  2. 2.University of Chinese Academy of sciencesBeijingPeople’s Republic of China
  3. 3.Chengdu Institute for Food and Drug ControlChengduPeople’s Republic of China

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