Dummy Molecularly Imprinted Matrix Solid-Phase Dispersion for Selective Extraction of Seven Estrogens in Aquatic Products

  • Jiwang TangEmail author
  • Jianxiu Wang
  • Liejiang Yuan
  • Yong Xiao
  • Shuxia Wang
  • Xiu Wang


A simple and sensitive method based on dummy molecular imprinting and matrix solid-phase dispersion (DMI–MSPD) was developed for the simultaneous detection of estrogens including estrone, 17β-estradiol, aestriol, ethinylestradiol, dienestrol, diethylstilbestrol, and hexestrol in aquatic products. The method was followed by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). In this method, the dummy molecularly imprinted polymers (DMIPs) were synthesized by Pickering emulsion polymerization, in which genistein was used as template molecular. According to the results of the experiment, the method of DMI–MSPD showed high affinity and a good selectivity for the seven estrogens in aquatic products. Compared with conventional MSPD sorbents, including C18, Florisil, and silica gel, the DMIPs could not only selectively elute the seven estrogens from aquatic products but also reduce matrix disruptions. The mean recovery rates of seven estrogens ranged from 89 to 103%, with relative standard deviations less than 5%. The limits of detection (LOD) and limits of quantitation (LOQ) were 1.6–2.8 ng g−1 and 5.4–7.6 ng g−1, respectively. Therefore, the proposed method can be well applied to the detection of estrogens in aquatic products.


Dummy molecularly imprinted polymers Matrix solid-phase dispersion Estrogen Aquatic products 



This study was funded by the Science And Technology Planning Project of Administration of Quality Supervision, Inspection and Quarantine of China (2017QK153).

Compliance of Ethical Standards

Conflict of Interest

Jiwang Tang declares that he has no conflict of interest. Jianxiu Wang declares that he has no conflict of interest. Liejiang Yuan declares that he has no conflict of interest. Yong Xiao declares that he has no conflict of interest. Shuxia Wang declares that she has no conflict of interest. Xiu wang declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1575_MOESM1_ESM.docx (558 kb)
ESM 1 (DOCX 557 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina
  2. 2.Hunan Testing Institute of Product and Commodity SupervisionChangshaChina

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