Disposable Pipette Extraction (DPX) Coupled with Liquid Chromatography–Tandem Mass Spectrometry for the Simultaneous Determination of Pesticide Residues in Wine Samples

  • Hua Zhang
  • Yuanyuan Li
  • Jing Zhu
  • Heng Li
  • Dehai Li
  • Zhaoxiang Liu
  • Xufang Sun
  • Baocheng WangEmail author
  • Qian WangEmail author
  • Yunfei GaoEmail author


A novel multiresidue analytical method based on disposable pipette extraction (DPX) to determine the trace level of 28 pesticides in wine samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed. The DPX method is used for both extraction and purification. After the optimization, targets were eluted with 1% HCl in acetonitrile and the eluent was placed into LC analysis. The developed method exhibited good linearity (R2 > 0.99) for all 28 pesticides. Satisfactory recovery of 28 pesticides was observed ranging from 71 to 109%, with the intraday relative standard deviations (RSDs) and inter-day RSDs of 1–11% and 3–18%, respectively. Limits of detection (LODs) and limits of quantification (LOQs) were found to range from 1.5 to 3 μg/L and 5 to 10 μg/L, respectively. DPX was more suitable as compared with quick, easy, cheap, effective, rugged, and safe (QuEChERS) and solid-phase extraction (SPE) methods. The DPX method was applied to 47 commercial wine samples collected from China, and pesticide residues (0.02–0.09 mg/L) were found. In general, the developed method was highly reliable, selective, and sensitive for analyzing 28 pesticide residues in wine samples and would be applicable to other beverages.


Disposable pipette extraction Pesticide residues LC–MS/MS Wine 



This study was funded by the National Natural Science Foundation of China (grant 31800721; 31470872), and the Science and Technology Program of Guangzhou (grant 201604020162).

Compliance with Ethical Standards

Conflict of Interest

Hua Zhang declares that she has no conflict of interest. Yuanyuan Li declares that she has no conflict of interest. Jing Zhu declares that she has no conflict of interest. Heng Li declares that he has no conflict of interest. Dehai Li declares that he has no conflict of interest. Zhaoxiang Liu declares that he has no conflict of interest. Xufang Sun declares that he has no conflict of interest. Baocheng Wang declares that he has no conflict of interest. Qian Wang declares that she has no conflict of interest. Yunfei Gao declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2019_1569_MOESM1_ESM.docx (430 kb)
ESM 1 (DOCX 430 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Zhuhai Precision Medical Center, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University)Jinan UniversityZhuhaiPeople’s Republic of China
  2. 2.The Biomedical Translational Research InstituteJinan University Faculty of Medical Science, Jinan UniversityGuangzhouPeople’s Republic of China
  3. 3.Department of Orthopedics, First Affiliated HospitalJinan UniversityGuangzhouPeople’s Republic of China
  4. 4.Department of Intensive Care UnitJinan Central Hospital Affiliated to Shandong UniversityJinanPeople’s Republic of China
  5. 5.Tsinghua Berkeley Shenzhen InstituteTsinghua UniversityShenzhenPeople’s Republic of China

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