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Evaluation of Dispersive Solid-Phase Extraction (d-SPE) as a Clean-up Step for Phenolic Compound Determination of Myrciaria cauliflora Peel

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Abstract

In this study, dispersive solid-phase extraction (d-SPE) was evaluated as a clean-up step for the determination of phenolic compounds in Myrciaria cauliflora peel using an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method. For the d-SPE clean-up step, the sorbents, diatomaceous earth (DE), chitosan (CT), and graphitized carbon black (GCB), were evaluated in different proportions. The d-SPE clean-up step efficiency was evaluated through total anthocyanin content (ACC) and spectrophotometric scanning of the extracts. The use of DE with GCB in a ratio of 100:10 (m/m) was the best clean-up step condition. The ACC showed an 83% reduction of the amount of anthocyanins, and the scanning spectra confirmed that all extracts submitted to the clean-up step presented pigment reduction. A total of eight compounds were found in M. cauliflora peel, with the highest abundance of ellagic acid (1643 ± 353 mg kg−1), gallic acid (252 ± 10 mg kg−1), and 4-hydroxybenzoic acid (154 ± 133 mg kg−1), indicating the antioxidant potential of the peel, which is frequently considered a by-product of Myrciaria cauliflora fruits.

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Correspondence to Carlos Eduardo Rubio Senes.

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Carlos Eduardo Rubio Senes declares that he has no conflict of interest. Antonio Eduardo Nicácio declares that he has no conflict of interest. Carina Alexandra Rodrigues declares that she has no conflict of interest. Luciana Pelissari Manin declares that she has no conflict of interest. Liane Maldaner declares that she has no conflict of interest. Jesuí Vergílio Visentainer declares that he has no conflict of interest.

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Senes, C.E.R., Nicácio, A.E., Rodrigues, C.A. et al. Evaluation of Dispersive Solid-Phase Extraction (d-SPE) as a Clean-up Step for Phenolic Compound Determination of Myrciaria cauliflora Peel. Food Anal. Methods 13, 155–165 (2020) doi:10.1007/s12161-019-01566-9

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Keywords

  • UHPLC-MS/MS
  • Anthocyanins
  • Sorbent
  • Jabuticaba
  • d-SPE
  • Clean-up