Measuring Primary Lipid Oxidation in Food Products Enriched with Colored Microalgae
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Microalgae are a valuable alternative source of n-3 LC-PUFA and have already proven their potential in different food products. However, enrichment of food products with n-3 LC-PUFA implies an increased sensitivity to lipid oxidation. Numerous analytical techniques have already been developed to determine and follow lipid oxidation. Photoautotrophic microalgae often contain, besides n-3 LC-PUFA, other compounds like carotenoids and chlorophylls. These colored compounds may interfere with the standard analytical techniques. This study contributes to optimize a simple and low-cost method to measure the degree of primary lipid oxidation in food products enriched with photoautotrophic microalgae. The standard iodometric titration and three spectrophotometric methods (FOX, IDF, and CD & CT) were investigated. The FOX method was selected as the preferable method, although interference due to the presence of metal ions could occur. This could partially be solved by a supplemental step with TPP addition. However, as this additional step did not change the trend in oxidative values during storage, it was suggested to normalize the values of the FOX method to week zero to investigate the trend of oxidation during storage of products enriched with photoautotrophic microalgae.
KeywordsPhotoautotrophic microalgae Colored food product Peroxide value Lipids Hydroperoxide determination
- CD & CT
Conjugated dienes & conjugated trienes
- FOX method
Ferrous oxidation–xylenol orange method
- IDF method
Ferric thiocyanate-based International Dairy Federation method
- n-3 LC-PUFA
Omega-3 long chain polyunsaturated fatty acids
We want to thank K. Raes (Ghent University Campus Kortrijk, Belgium) for the support with the ICP-OES analysis.
The research presented in this paper was financially supported by the Research Foundation - Flanders (FWO SB PhD fellowship Lore Gheysen 1S 151287 16N and Tom Bernaerts 1S 099 16N) and Industrial Research Fund KULeuven (IOF-KP Vegetalgae).
Compliance with Ethical Standards
Conflict of Interest
Lore Gheysen declares that she has no conflict of interest. Céline Dejonghe declares that she has no conflict of interest. Tom Bernaerts declares that he has no conflict of interest. Ann Van Loey declares that she has no conflict of interest. Luc De Cooman declares that he has no conflict of interest. Imogen Foubert declares that she has no conflict of interest.
No conflicts, informed consent, human or animal rights applicable.
- AOCS (2003) Official methods and recommended practices of the AOCS, 5th edn. American Oil Chemists’ Society, ChampaignGoogle Scholar
- Farhoosh R, Moosavi SMR (2009) Evaluating the performance of peroxide and conjugated diene values in monitoring quality of used frying oils. J Agric Sci Technol 11:173–179Google Scholar
- Gay C, Collins J, Gebicki JM (1999) Determination of iron in solutions with the ferric – xylenol orange complex. 148:143–148Google Scholar
- Gheysen L, Matton V, Foubert I (2018a) Microalgae as a source of omega-3 polyunsaturated fatty acids. In: Catala A (ed) Polyunsaturated fatty acids (PUFAs): food sources, health effects and significance in biochemistry. Nova Science Publishers, New YorkGoogle Scholar
- Jacobsen C, Sørensen ADM, Nielsen NS (2013) Stabilization of omega-3 oils and enriched foods using antioxidants, food enrichment with omega-3 fatty acids. Woodhead Publishing LimitedGoogle Scholar
- Mizuguchi H, Yotsuyanagi T (2001) Visual threshold detection of trace metal ions using a bi-functional metallochromic reagent. Anal Sci 17:1687–1689Google Scholar
- Nourooz-Zadeh J, Tajaddini-Sarmadi J, Wolff SP (1994) Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Anal BiochemGoogle Scholar
- Schaich KM (2016) Chapter 1 – Analysis of lipid and protein oxidation in fats, oils, and foods, oxidative stability and shelf life of foods containing oils and fats. Elsevier IncGoogle Scholar
- Schaich KM, Shahidi F, Zhong Y, Eskin NAM (2013) Lipid oxidation, Third Edit. ed, Biochemistry of Foods. ElsevierGoogle Scholar
- Shahidi F, Zhong Y (2005) Lipid oxidation: measurement methods. Bailey’s Ind. Oil fat prodGoogle Scholar
- Shantha NC, Decker EA (1994) Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxides values of food lipids. J AOAC Int 77:421–424Google Scholar
- Wang N, Ma T, Yu X, Xu L, Zhang R (2016) Determination of peroxide values of edible oils by ultraviolet spectrometric method. Food anal. Methods 9:1412–1417Google Scholar
- Wrolstad RE, Acree TE, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Smith DM, Sporns P (2005) Handbook of food analytical chemistry, volume 1 : water, protein, enzymes, lipids, and carbohydrates. Handb Food Anal ChemGoogle Scholar