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Food Analytical Methods

, Volume 12, Issue 10, pp 2150–2160 | Cite as

Measuring Primary Lipid Oxidation in Food Products Enriched with Colored Microalgae

  • Lore GheysenEmail author
  • Céline Dejonghe
  • Tom Bernaerts
  • Ann Van Loey
  • Luc De Cooman
  • Imogen Foubert
Article
  • 55 Downloads

Abstract

Microalgae are a valuable alternative source of n-3 LC-PUFA and have already proven their potential in different food products. However, enrichment of food products with n-3 LC-PUFA implies an increased sensitivity to lipid oxidation. Numerous analytical techniques have already been developed to determine and follow lipid oxidation. Photoautotrophic microalgae often contain, besides n-3 LC-PUFA, other compounds like carotenoids and chlorophylls. These colored compounds may interfere with the standard analytical techniques. This study contributes to optimize a simple and low-cost method to measure the degree of primary lipid oxidation in food products enriched with photoautotrophic microalgae. The standard iodometric titration and three spectrophotometric methods (FOX, IDF, and CD & CT) were investigated. The FOX method was selected as the preferable method, although interference due to the presence of metal ions could occur. This could partially be solved by a supplemental step with TPP addition. However, as this additional step did not change the trend in oxidative values during storage, it was suggested to normalize the values of the FOX method to week zero to investigate the trend of oxidation during storage of products enriched with photoautotrophic microalgae.

Keywords

Photoautotrophic microalgae Colored food product Peroxide value Lipids Hydroperoxide determination 

Abbreviations

CD & CT

Conjugated dienes & conjugated trienes

DHA

Docosahexaenoic acid

EPA

Eicosapentaenoic acid

FOX method

Ferrous oxidation–xylenol orange method

IDF method

Ferric thiocyanate-based International Dairy Federation method

n-3 LC-PUFA

Omega-3 long chain polyunsaturated fatty acids

TPP

Triphenylphosphine

Notes

Acknowledgments

We want to thank K. Raes (Ghent University Campus Kortrijk, Belgium) for the support with the ICP-OES analysis.

Funding

The research presented in this paper was financially supported by the Research Foundation - Flanders (FWO SB PhD fellowship Lore Gheysen 1S 151287 16N and Tom Bernaerts 1S 099 16N) and Industrial Research Fund KULeuven (IOF-KP Vegetalgae).

Compliance with Ethical Standards

Conflict of Interest

Lore Gheysen declares that she has no conflict of interest. Céline Dejonghe declares that she has no conflict of interest. Tom Bernaerts declares that he has no conflict of interest. Ann Van Loey declares that she has no conflict of interest. Luc De Cooman declares that he has no conflict of interest. Imogen Foubert declares that she has no conflict of interest.

Ethical Approval

No conflicts, informed consent, human or animal rights applicable.

Informed Consent

Not applicable.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Research Unit Food & LipidsKU Leuven KulakKortrijkBelgium
  2. 2.Leuven Food Science and Nutrition Research Centre (LFoRCe)KU LeuvenLeuvenBelgium
  3. 3.Laboratory of Food TechnologyKU LeuvenLeuvenBelgium
  4. 4.Laboratory of Enzyme, Fermentation and Brewing TechnologyKU Leuven Technology Campus GhentGhentBelgium

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