Automatically High-Throughput Quantification by Paper Spray Ionization Mass Spectrometry for Multiple Pesticides in Wine

  • Tianyang Guo
  • Wei Yong
  • Yiyang DongEmail author


A rapid paper spray (PS) ionization tandem mass spectrometry analytical method has been developed to determine multiple pesticides in red wine. Without chromatographic separation, 36 pesticides in wine could be simultaneously determined less than 9 and 3 min, followed by off-line QuEChERS treatment and on-line paper adsorption treatment, respectively. Isotopically labeled internal standard (IS) was utilized to calibrate injection process, and linear ranges were one to three orders of magnitude for different pesticides. LODs were ranging between 0.1 and 100 ng/mL, and LOQs were ranging between 0.3 and 300 ng/mL. Method validation was used to evaluate the accuracy and precision, and the results for most analytes were at the range of 75–120%. All the data processes were automatically achieved by build-in instrument software. These methods were also compared with QuEChERS-DART-MS/MS and DART-MS/MS. The results demonstrated that PS-MS/MS was a rapid and simple method to quantify multiple pesticides in red wine.


Automatically high-throughput quantification Paper spray (PS) ionization Tandem mass spectrometry Pesticide Red wine QuEChERS Paper adsorption Ambient Rapid 



Authors wish to thank the support of Tianyang Guo through the CSC Scholarship Program from BUCT.

Authors are also grateful to R. Graham Cooks, Karen E. Yannell, Qiang Ma, Fan Pu, and Pingping Fang for helpful advice.


This study was funded by the National Key R&D Program of China (2016YFF0203703), the Key Program of a Synchronized Detection Research and Risk Assessment for Radionuclides in Foods of Beijing Municipal Science & Technology Commission (D161100002116002), and NSFC grant (51673012).

Compliance with Ethical Standards

Conflict of Interest

Tianyang Guo declares that she has no conflict of interest. Wei Yong declares that he has no conflict of interest. Yiyang Dong declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals.

Informed Consent

Not applicable.

Supplementary material

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ESM 1 (DOCX 31 kb)
12161_2019_1450_MOESM2_ESM.docx (32 kb)
ESM 2 (DOCX 32 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Beijing Higher Institution Engineering Research Center of Food Additives and IngredientsBeijing Technology and Business UniversityBeijingChina
  2. 2.College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijingChina
  3. 3.Institute of Food SafetyChinese Academy of Inspection and QuarantineBeijingChina

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