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Simultaneous Detection of Listeria monocytogenes and Salmonella typhimurium by a SERS-Based Lateral Flow Immunochromatographic Assay

  • Zhengzong WuEmail author
Article
  • 27 Downloads

Abstract

Foodborne diseases arise from Listeria monocytogenes (L. monocytogenes) and Salmonella typhimurium (S. typhimurium) are global public health problems, influencing both developing and developed countries. Therefore, their rapid and accurate detection is of much concern in food safety and point-of-care diagnostics. In this study, a surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for simultaneous detection of them was developed. Under optimal conditions, the proposed assay showed good linear responses in the ranges of 102–107 cfu/mL and 102–107 cfu/mL with limits of detection of 75 cfu/mL and 75 cfu/mL, respectively, for L. monocytogenes and S. typhimurium, respectively. The practicality of the LFA in real food sample (milk) was also validated. Overall, the proposed assay is promising and has a good chance to be employed for multiple pathogen detection in food products.

Keywords

Food analysis Surface-enhanced Raman scattering Multiplex detection Pathogen Lateral flow 

Notes

Funding Information

This research was supported by the Postgraduate research and Practice Innovation Program of Jiangsu Province (No. KYCX18_751), Nature Science Foundation of Jiangsu Province (No. BK20160168), Nature Science Foundation of China (Nos. 31601413 and 31501418) and Special Funds for Taishan Scholars Project.

Compliance with Ethical Standards

Conflict of Interest

Zhengzong Wu declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

References

  1. Cho IH, Irudayaraj J (2013) Lateral-flow enzyme immunoconcentration for rapid detection of Listeria monocytogenes. Anal Bioanal Chem 405:3313–3319CrossRefGoogle Scholar
  2. Cocolin L, Manzano M, Cantoni C, Comi G (2010) Use of polymerase chain reaction and restriction enzyme analysis to directly detect and identify Salmonella typhimurium in food. J Appl Microbiol 85:673–677CrossRefGoogle Scholar
  3. Di Nardo F, Alladio E, Baggiani C, Cavalera S, Giovannoli C, Spano G, Anfossi L (2019) Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line. Talanta 192:288–294CrossRefGoogle Scholar
  4. Hwang J, Lee S, Choo J (2016) Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B. Nanoscale 8:11418–11425CrossRefGoogle Scholar
  5. Karim MN, Anderson SR, Singh S, Ramanathan R, Bansal V (2018) Nanostructured silver fabric as a free-standing NanoZyme for colorimetric detection of glucose in urine. Biosens Bioelectron 110:8–15.  https://doi.org/10.1016/j.bios.2018.03.025 CrossRefGoogle Scholar
  6. Kolosova AY, Saeger SD, Sibanda L, Verheijen R, Peteghem CV (2007) Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol. Anal Bioanal Chem 389:2103–2107CrossRefGoogle Scholar
  7. Lan Y-b, S-z W, Y-g Y, Hoffmann WC, X-z Z (2008) Using a surface plasmon resonance biosensor for rapid detection of Salmonella Typhimurium in chicken carcass. J Bionic Eng 5:239–246.  https://doi.org/10.1016/S1672-6529(08)60030-X CrossRefGoogle Scholar
  8. Li C-z et al (2011) Paper based point-of-care testing disc for multiplex whole cell bacteria analysis. Biosens Bioelectron 26:4342–4348.  https://doi.org/10.1016/j.bios.2011.04.035 CrossRefGoogle Scholar
  9. Li X-M, Zhu J, Pan Y, Meng R, Zhang B, Chen H-Q (2019) Fabrication and characterization of Pickering emulsions stabilized by octenyl succinic anhydride-modified gliadin nanoparticle. Food Hydrocoll 90:19–27CrossRefGoogle Scholar
  10. Lin LK, Stanciu L (2018) Bisphenol A detection using gold nanostars in a SERS improved lateral flow immunochromatographic assay. Sensors Actuators B Chem 276:222–229CrossRefGoogle Scholar
  11. Liu H-b, X-j D, Zang Y-X, Li P, Wang S (2017) SERS-based lateral flow strip biosensor for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. J Agric Food Chem 65:10290–10299.  https://doi.org/10.1021/acs.jafc.7b03957 CrossRefGoogle Scholar
  12. Liu P, Kang X, Cui B, Wang R, Wu Z (2019) Effects of glycerides with different molecular structures on the properties of maize starch and its film forming capacity. Ind Crop Prod 129:512–517CrossRefGoogle Scholar
  13. Ma W-q, Fang Y, G-l H, W-g W (2010) Adsorption behaviors of 4-mercaptobenzoic acid on silver and gold films. Chin J Chem Phys 23:659–663CrossRefGoogle Scholar
  14. Ma Y-S, Pan Y, Xie Q-T, Li X-M, Zhang B, Chen H-Q (2019) Evaluation studies on effects of pectin with different concentrations on the pasting, rheological and digestibility properties of corn starch. Food Chem 274:319–323.  https://doi.org/10.1016/j.foodchem.2018.09.005 CrossRefGoogle Scholar
  15. Magliulo M, Simoni P, Guardigli M, Michelini E, Luciani M, Rossella Lelli A, Roda A (2007) A rapid multiplexed chemiluminescent immunoassay for the detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes Pathogen Bacteria. J Agric Food Chem 55:4933–4939CrossRefGoogle Scholar
  16. Mascini M, Guilbault GG, Monk IR, Hill C, Carlo MD, Compagnone D (2008) Screening of rationally designed oligopeptides for Listeria monocytogenes detection by means of a high density colorimetric microarray. Microchim Acta 163:227–235CrossRefGoogle Scholar
  17. Nguyen P-D, Tran TB, Nguyen DTX, Min J (2014) Magnetic silica nanotube-assisted impedimetric immunosensor for the separation and label-free detection of Salmonella typhimurium. Sensors Actuators B Chem 197:314–320.  https://doi.org/10.1016/j.snb.2014.02.089 CrossRefGoogle Scholar
  18. Oravec M, Sasinková V, Tomanová K, Gál L, Parciová S, Huck CW (2018) In-situ surface-enhanced Raman scattering and FT-Raman spectroscopy of black prints. Vib Spectrosc 94:16–21CrossRefGoogle Scholar
  19. Quesada-González D, Merkoçi A (2015) Nanoparticle-based lateral flow biosensors. Biosens Bioelectron 73:47–63.  https://doi.org/10.1016/j.bios.2015.05.050 CrossRefGoogle Scholar
  20. Salam F, Uludag Y, Tothill IE (2013) Real-time and sensitive detection of Salmonella Typhimurium using an automated quartz crystal microbalance (QCM) instrument with nanoparticles amplification. Talanta 115:761–767CrossRefGoogle Scholar
  21. Srisa-Art M, Boehle KE, Geiss BJ, Henry CS (2018) Highly sensitive detection of Salmonella typhimurium using a colorimetric paper-based analytical device coupled with immunomagnetic separation. Anal Chem 90:1035–1043.  https://doi.org/10.1021/acs.analchem.7b04628 CrossRefGoogle Scholar
  22. Techathuvanan C, Draughon FA, D’Souza DH (2011) Comparison of reverse transcriptase PCR, reverse transcriptase loop-mediated isothermal amplification, and culture-based assays for Salmonella detection from pork processing environments. J Food Prot 74:294–301CrossRefGoogle Scholar
  23. Thi MD, Volka K (2010) Surface-enhanced Raman spectroscopic and surface plasmon resonance in situ study of self-assembly of 4-mercaptobenzoic acid on gold surface. J Mol Struct 976:297–300.  https://doi.org/10.1016/j.molstruc.2010.04.011 CrossRefGoogle Scholar
  24. Tnt D, Lee EY, Koo B, Jin CE, Lee TY, Shin Y (2017) A microfluidic enrichment platform with a recombinase polymerase amplification sensor for pathogen diagnosis. Anal Biochem 544:87–92Google Scholar
  25. Tully E, Hearty S, Leonard P, O’Kennedy R (2006) The development of rapid fluorescence-based immunoassays, using quantum dot-labelled antibodies for the detection of Listeria monocytogenes cell surface proteins. Int J Biol Macromol 39:127–134.  https://doi.org/10.1016/j.ijbiomac.2006.02.023 CrossRefGoogle Scholar
  26. Vaughan RD, O’Sullivan CK, Guilbault GG (2001) Development of a quartz crystal microbalance (QCM) immunosensor for the detection of Listeria monocytogenes. Enzym Microb Technol 29:635–638CrossRefGoogle Scholar
  27. Wang X, Choi N, Cheng Z, Ko J, Chen L, Choo J (2017) Simultaneous detection of dual nucleic acids using a SERS-based lateral flow assay biosensor. Anal Chem 89:1163–1169CrossRefGoogle Scholar
  28. Wernars K, Heuvelman CJ, Chakraborty T, Notermans SH (2010) Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. J Appl Microbiol 70:121–126Google Scholar
  29. Wu S, Zhang H, Shi Z, Duan N, Fang CC, Dai S, Wang Z (2015) Aptamer-based fluorescence biosensor for chloramphenicol determination using upconversion nanoparticles. Food Control 50:597–604CrossRefGoogle Scholar
  30. Wu Y, Guo S, Dong Q, Song Y (2016) Development of an immunochromatographic test strip for rapid simultaneous detection of enrofloxacin and ofloxacin in tissue of chicken muscle and pork. Food Anal Methods 9:2807–2813.  https://doi.org/10.1007/s12161-016-0474-x CrossRefGoogle Scholar
  31. Wu Z, Xu E, Jin Z, Irudayaraj J (2018a) An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection. Food Chem 249:136–142CrossRefGoogle Scholar
  32. Wu Z, He D, Xu E, Jiao A, Chughtai MFJ, Jin Z (2018b) Rapid detection of β-conglutin with a novel lateral flow aptasensor assisted by immunomagnetic enrichment and enzyme signal amplification. Food Chem 269:375–379.  https://doi.org/10.1016/j.foodchem.2018.07.011 CrossRefGoogle Scholar
  33. Xie Z, Wang Y, Chen Y, Xu X, Jin Z, Ding Y, Yang N, Wu F (2017) Tuneable surface enhanced Raman spectroscopy hyphenated to chemically derivatized thin-layer chromatography plates for screening histamine in fish. Food Chem 230:547–552CrossRefGoogle Scholar
  34. Yang L, Li Y (2006) Simultaneous detection of Escherichia coli O157:H7 and Salmonella Typhimurium using quantum dots as fluorescence labels. Analyst 131:394–401CrossRefGoogle Scholar
  35. Yang J et al. (2014) Guided-mode resonance grating with self-assembled silver nanoparticles for surface-enhanced Raman scattering spectroscopy. Photonics 1:380–389.  https://doi.org/10.3390/photonics1040380 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biobased Material and Green PapermakingQilu University of Technology, Shandong Academy of SciencesJinanChina
  2. 2.School of Food Science and EngineeringQilu University of Technology, Shandong Academy of SciencesJinanChina

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