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Micro-scaled Quantitative Method to Analyze Olive Oil Polyphenols

  • Giovanni Caprioli
  • Maria Chiara Boarelli
  • Massimo Ricciutelli
  • Gianni Sagratini
  • Dennis FioriniEmail author
Article
  • 11 Downloads

Abstract

This study aims to improve an analytical method to quantify phenolic substances in olive oil. In order to minimize time required and quantity of solvents, sample extract preparation performed for a previously developed high-performance liquid chromatography–diode array detector to quantify olive oil polyphenols has been ten times downscaled and then validated. The new method performs the extraction of phenolic substances from 0.5 g of oil and allows to quantify the phenolic acids vanillic acid, p-coumaric acid, and ferulic acid; the phenolic alcohols tyrosol and hydroxytyrosol; secoiridoid derivatives; the lignans pinoresinol and acetoxypinoresinol; and the flavonoids luteolin and apigenin. Recoveries obtained were 66–89% for phenolic alcohols, 64–90% for phenolic acids, 93–96% for oleuropein (used as a reference for secoiridoid derivatives), 71–95% for flavonoids, and 97–100% for lignans. The total quantity of organic solvents used in the sample preparation is decreased from 30 to 3 mL with an important abatement of waste, costs, and working time requested.

Keywords

Olive oil polyphenols Quantitative determination HPLC-DAD Secoiridoid derivatives Green method 

Notes

Funding

This study was funded by the University of Camerino (grant “Fondo di Ateneo per la Ricerca” 2014–2015, project: “AEVOO: Authentication of Extra Virgin Olive Oil”).

Compliance with Ethical Standards

Conflict of Interest

Giovanni Caprioli declares that he has no conflict of interest. Maria Chiara Boarelli declares that she has no conflict of interest. Massimo Ricciutelli declares that he has no conflict of interest. Gianni Sagratini declares that he has no conflict of interest. Dennis Fiorini declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of PharmacyUniversity of CamerinoCamerinoItaly
  2. 2.School of Science and Technology, Chemistry DivisionUniversity of CamerinoCamerinoItaly
  3. 3.HPLC-MS LaboratoryUniversity of CamerinoCamerinoItaly

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