Food Analytical Methods

, Volume 12, Issue 3, pp 687–696 | Cite as

Silane Coupling Agents Modified Silica and Graphene Oxide Materials for Determination of Sulfamerazine and Sulfameter in Milk by HPLC

  • Yaying Lv
  • Qilin Deng
  • Kyung Ho Row
  • Tao ZhuEmail author


Silica and graphene oxide were separately modified by using three different kinds of silane coupling agents, including (3-chloropropyl)-trimethoxysilane (NQ54), 3-methacryloxypropyltrimethoxysilane (KH570), and (3-aminopropyl) triethoxysilane (KH550). The characterization of obtained materials was tested by Fourier transform infrared spectroscopy and scanning electron microscopy, and the electronegativity was determined by Zetasizer Nano ZSP. The obtained materials were used to determine the sulfamerazine and sulfameter by ultrasonic-assisted dispersive solid-phase extraction. The static sorption experiment showed that the graphene oxide modified by NQ54 had a good sorption ability for two targets. Under the optimal condition, a reliable analytical method was developed for high recognition towards two targets in milk by NQ54-modified graphene oxide with satisfactory extraction recoveries (sulfamerazine 96.75% and sulfameter 94.08%, respectively). The recoveries of proposed method at three spiked levels were 92.16–101.71% and 92.95–103.81%, respectively, with the relative standard deviation less than 3.20%. It was indicated that graphene oxide modified by NQ-54 showed better performance for two targets, and the proposed method had been successfully applied for the determination of sulfonamides sample in milk.


Terminal chlorine Graphene oxide Silane coupling agent Sulfonamides 



This study was supported by the National Natural Science Foundation of China (21808173) and Training Project of Innovation Team of Colleges and Universities in Tianjin (TD13-5020). It was also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1A4A1042434).

Compliance with Ethical Standards

Conflict of Interest

Yaying Lv declares that he has no conflict of interest. Qilin Deng declares that she has no conflict of interest. Kyung Ho Row declares that she has no conflict of interest. Tao Zhu declares that she has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Informed consent is not applicable in this study.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical EngineeringTianjin University of TechnologyTianjinChina
  2. 2.Department of Chemistry and Chemical EngineeringInha UniversityIncheonSouth Korea

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