Food Analytical Methods

, Volume 12, Issue 3, pp 742–751 | Cite as

Determination of Valifenalate in Grape, Vegetables, and Soil Using Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry and Exploration of Its Degradation Behavior in Grape Field

  • Runan Li
  • Zenglong Chen
  • Yan Tao
  • Xinglu Pan
  • Duoduo Jiang
  • Fengshou DongEmail author
  • Jun Xu
  • Xingang Liu
  • Xiaohu Wu
  • Yongquan Zheng


A novel, sensitive, and rapid analytical method for analyzing valifenalate residue in grape, vegetables (tomato, lettuce, eggplant, potato), and soil was established and validated using a modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method by ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The determination of the analyte was achieved within 4 min for per injection using electrospray ionization (ESI) operated in positive ion mode, and the retention time was 1.92 min. The developed method exhibited good linearity (R2 ≥ 0.9941) and the limit of quantitation (LOQ) was 0.5 μg/kg for analyte in selected matrices. The mean recoveries in six matrices ranged from 74.5 to 106.6% with intra-day relative standard deviation (RSD) of 0.7–12.3% and inter-day RSD of 3.2–12.2% at four spiking levels (0.5, 10, 100, 500 μg/kg) in recovery assays. The proposed method was applied to explore the dissipation rates of valifenalate in grapes and soil under field conditions, and the half-lives of analyte in grape and soil were 15.5 ± 1.4 and 3.8 ± 0.1 days, respectively. The study indicated that the method was effective and reliable for detection of valifenalate residues and the dissipation data can provide reference towards safety application for the analyte.


Valifenalate Residues Degradation rate Ultrahigh performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) 



This work was financially supported by the National Key Research and Development Program of China (2016YFD0200204).

Compliance with Ethical Standards

The article is an original research which has neither been published previously nor submitted to more than one journal for simultaneous consideration.

Conflict of Interest

Runan Li declares that he has no conflict of interest. Zenglong Chen declares that he has no conflict of interest. Yan Tao declares that he has no conflict of interest. Xinglu Pan declares that he has no conflict of interest. Duoduo Jiang declares that he has no conflict of interest. Fengshou Dong declares that he has no conflict of interest. Jun Xu declares that he has no conflict of interest. Xingang Liu declares that he has no conflict of interest. Xiaohu Wu declares that he has no conflict of interest. Yongquan Zheng declares that he has no conflict of interest.

Ethical Approval

The article does not contain any studies with human participants or animal subjects.

Informed Consent

All the authors named in a manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of ZoologyChinese Academy of SciencesBeijingPeople’s Republic of China

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