Amperometric Determination of Glucose in White Grape and in Tablets as Ingredient by Screen-Printed Electrode Modified with Glucose Oxidase and Composite of Platinum and Multiwalled Carbon Nanotubes
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Multiwalled carbon nanotubes (MWCNTs) and nanocomposite made from MWCNTs and Pt nanoparticles (Pt-MWCNTs) were used as bulk modifiers of screen-printed carbon electrodes (SPCEs). Cyclic voltammetric and amperometric measurements were performed by the homemade substrate electrodes, the bare SPCE and the modified ones (MWCNT-SPCE and Pt-MWCNT-SPCE), with aim to investigate their applicability for determination of H2O2 in phosphate buffer solution (0.1 M; pH 7.50) as supporting electrolyte. Pt-MWCNT-SPCE showed improved analytical performances including the significant decrease in overpotential of H2O2 compared to SPCE and MWCNT-SPCE. So, Pt-MWCNT-SPCE served as substrate electrode for design of a simple first-generation biosensor made from glucose oxidase (GOx) and Nafion® forming the GOx/Pt-MWCNT-SPCE for reliable determination of glucose via its enzymatic by-product, the H2O2. The surface morphology characterization by scanning electron microscopy confirmed the presence of immobilized spherical shaped biosensing units at the GOx/Pt-MWCNT-SPCE. In the case of amperometry of glucose, optimal potential for the working electrode was − 0.50 V vs. SCE, and a satisfactory linearity was obtained in the tested concentration range from 65.8 to 260.6 μg mL−1, with estimated LOQ of 35.0 μg mL−1. The developed cost-effective amperometric method in combination with GOx/Pt-MWCNT-SPCE was successfully applied for the determination of glucose in selected food samples: white grapes and glucose tablets. The obtained results were in good agreement with those received by the alternatively used commercially available glucometer and by the producer declared value in the case of the tablet sample as well.
KeywordsScreen-printed carbon electrode Platinum and multiwalled carbon nanotube composite mediator Glucose oxidase Amperometry Glucose determination Food samples
GV thanks the Domus Hungarica Scientiarum et Artium fellowship.
All authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.
This study received a financial support from the Ministry of Science and Technological Development of the Republic of Serbia (Project Nos. 172059 and 172012) and CEEPUSIII (CZ-0212-09-1718) network.
Compliance with Ethical Standards
Conflict of Interest
Valéria Guzsvány declares that she has no conflict of interest. Jasmina Anojčić declares that she has no conflict of interest. Olga Vajdle declares that she has no conflict of interest. Emil Radulović declares that he has no conflict of interest. Dániel Madarász declares that he has no conflict of interest. Zoltán Kónya declares that he has no conflict of interest. Kurt Kalcher declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors. This is an original research article that has neither been published previously nor considered presently for publication elsewhere.
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