Food Analytical Methods

, Volume 12, Issue 2, pp 570–580 | Cite as

Amperometric Determination of Glucose in White Grape and in Tablets as Ingredient by Screen-Printed Electrode Modified with Glucose Oxidase and Composite of Platinum and Multiwalled Carbon Nanotubes

  • Valéria GuzsványEmail author
  • Jasmina Anojčić
  • Olga Vajdle
  • Emil Radulović
  • Dániel Madarász
  • Zoltán Kónya
  • Kurt Kalcher


Multiwalled carbon nanotubes (MWCNTs) and nanocomposite made from MWCNTs and Pt nanoparticles (Pt-MWCNTs) were used as bulk modifiers of screen-printed carbon electrodes (SPCEs). Cyclic voltammetric and amperometric measurements were performed by the homemade substrate electrodes, the bare SPCE and the modified ones (MWCNT-SPCE and Pt-MWCNT-SPCE), with aim to investigate their applicability for determination of H2O2 in phosphate buffer solution (0.1 M; pH 7.50) as supporting electrolyte. Pt-MWCNT-SPCE showed improved analytical performances including the significant decrease in overpotential of H2O2 compared to SPCE and MWCNT-SPCE. So, Pt-MWCNT-SPCE served as substrate electrode for design of a simple first-generation biosensor made from glucose oxidase (GOx) and Nafion® forming the GOx/Pt-MWCNT-SPCE for reliable determination of glucose via its enzymatic by-product, the H2O2. The surface morphology characterization by scanning electron microscopy confirmed the presence of immobilized spherical shaped biosensing units at the GOx/Pt-MWCNT-SPCE. In the case of amperometry of glucose, optimal potential for the working electrode was − 0.50 V vs. SCE, and a satisfactory linearity was obtained in the tested concentration range from 65.8 to 260.6 μg mL−1, with estimated LOQ of 35.0 μg mL−1. The developed cost-effective amperometric method in combination with GOx/Pt-MWCNT-SPCE was successfully applied for the determination of glucose in selected food samples: white grapes and glucose tablets. The obtained results were in good agreement with those received by the alternatively used commercially available glucometer and by the producer declared value in the case of the tablet sample as well.


Screen-printed carbon electrode Platinum and multiwalled carbon nanotube composite mediator Glucose oxidase Amperometry Glucose determination Food samples 



GV thanks the Domus Hungarica Scientiarum et Artium fellowship.

Author Contribution

All authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.

Funding Information

This study received a financial support from the Ministry of Science and Technological Development of the Republic of Serbia (Project Nos. 172059 and 172012) and CEEPUSIII (CZ-0212-09-1718) network.

Compliance with Ethical Standards

Conflict of Interest

Valéria Guzsvány declares that she has no conflict of interest. Jasmina Anojčić declares that she has no conflict of interest. Olga Vajdle declares that she has no conflict of interest. Emil Radulović declares that he has no conflict of interest. Dániel Madarász declares that he has no conflict of interest. Zoltán Kónya declares that he has no conflict of interest. Kurt Kalcher declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors. This is an original research article that has neither been published previously nor considered presently for publication elsewhere.

Informed Consent

Not applicable.


  1. Albanese D, Sannini A, Malvano F, Pilloton R, Di Matteo M (2014) Optimisation of glucose biosensors based on sol–gel entrapment and Prussian Blue-modified screen-printed electrodes for real food analysis. Food Anal Methods 7:1002–1008CrossRefGoogle Scholar
  2. Albareda-Sirvent M, Merkoci A, Alegret S (2000) Configurations used in the design of screen-printed enzymatic biosensors. A review. Sensors Actuators B Chem 69:153–163CrossRefGoogle Scholar
  3. Ammam M, Easton EB (2011) High-performance glucose sensor based on glucose oxidase encapsulated in new synthesized platinum nanoparticles supported on carbon Vulcan/Nafion composite deposited on glassy carbon. Sensors Actuators B Chem 155:340–346CrossRefGoogle Scholar
  4. Anojčić J, Guzsvány V, Vajdle O, Madarász D, Rónavári A, Kónya Z, Kalcher K (2016) Hydrodynamic chronoamperometric determination of hydrogen peroxide using carbon paste electrodes coated by multiwalled carbon nanotubes decorated with MnO2 or Pt particles. Sensors Actuators B Chem 233:83–92CrossRefGoogle Scholar
  5. Anojčić J, Guzsvány V, Vajdle O, Kónya Z, Kalcher K (2018) Rapid amperometric determination of H2O2 by a Pt nanoparticle/Vulcan XC72 composite-coated carbon paste electrode in disinfection and contact lens solutions. Monatsh Chem 149:1727–1738CrossRefGoogle Scholar
  6. Arduini F, Micheli L, Moscone D, Palleschi G, Piermarini S, Ricci F, Volpe G (2016) Electrochemical biosensors based on nanomodified screen-printed electrodes: recent applications in clinical analysis. Trends Anal Chem 79:114–126CrossRefGoogle Scholar
  7. Berisha L, Kalcher K, Hajrizi A, Arbneshi T (2013) A new biosensor for glucose based on screen printed carbon electrodes modified with tin(IV)-oxide. AJAC 4:27–35CrossRefGoogle Scholar
  8. Cash KJ, Clark HA (2010) Nanosensors and nanomaterials for monitoring glucose in diabetes. Trends Mol Med 16:584–593CrossRefGoogle Scholar
  9. Cardiel JJ, Zhao Y, Tonggu L, Wang L, Chung J-H, Shen AQ (2014) Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing. Lab Chip 14:3912–3916CrossRefGoogle Scholar
  10. Chang H-W, Tsai Y-C, Cheng C-W, Lin C-Y, Wu P-H (2013) Preparation of platinum/carbon nanotube in aqueous solution by femtosecond laser for non-enzymatic glucose determination. Sensors Actuators B Chem 183:34–39CrossRefGoogle Scholar
  11. Chen A, Chatterjee S (2013) Nanomaterials based electrochemical sensors for biomedical applications. Chem Soc Rev 42:5425–5438CrossRefGoogle Scholar
  12. Garcia-Perez T, Hong S-G, Kimb J, Ha S (2016) Entrapping cross-linked glucose oxidase aggregates within a graphitized mesoporous carbon network for enzymatic biofuel cells. Enzym Microb Technol 90:26–34CrossRefGoogle Scholar
  13. Guo MQ, Hong HS, Tang XN, Fang HD, Xu XH (2012) Ultrasonic electrodeposition of platinum nanoflowers and their application in non enzymatic glucose sensors. Electrochim Acta 63:1–8CrossRefGoogle Scholar
  14. Guzsvány V, Anojčić J, Radulović E, Vajdle O, Stanković I, Madarász D, Kónya Z, Kalcher K (2017) Screen-printed enzymatic glucose biosensor based on a composite made from multiwalled carbon nanotubes and palladium containing particles. Microchim Acta 184:1987–1996CrossRefGoogle Scholar
  15. Hrapovic S, Liu Y, Male KB, Luong JHT (2004) Electrochemical biosensing platforms using platinum nanoparticles and carbon nanotubes. Anal Chem 76:1083–1088CrossRefGoogle Scholar
  16. Jiang X, Wu Y, Mao X, Cui X, Zhu L (2011) Amperometric glucose biosensor based on integration of glucose oxidase with platinum nanoparticles/ordered mesoporous carbon nanocomposite. Sensors Actuators B Chem 153:158–163CrossRefGoogle Scholar
  17. Kalcher K, Svancara I, Buzuk M, Vytras K, Walcarius A (2009) Electrochemical sensors and biosensors based on heterogeneous carbon materials. Monatsh Chem 140:861–889CrossRefGoogle Scholar
  18. Li Z, Zhu Y, Zhang W, Xu C, Pan Y, Zhao Y (2017) A low-cost and high sensitive paper-based microfluidic device for rapid detection of glucose in fruit. Food Anal Methods 10:666–674CrossRefGoogle Scholar
  19. Luo X, Morrin A, Killard AJ, Smyth MR (2006) Application of nanoparticles in electrochemical sensors and biosensors. Electroanalysis 18:319–326CrossRefGoogle Scholar
  20. Luong JHT, Bouvrette P, Male KB (1997) Developments and applications of biosensors in food analysis. TIBTECH 15:369–377CrossRefGoogle Scholar
  21. Mello LD, Kubota LT (2002) Review of the use of biosensors as analytical tools in the food and drink industries. Food Chem 77:237–256CrossRefGoogle Scholar
  22. Monosik R, Stredansky M, Tkac J, Sturdik E (2012) Application of enzyme biosensors in analysis of food and beverages. Food Anal Methods 5:40–53CrossRefGoogle Scholar
  23. Muñoz-Robredo P, Robledo P, Manríquez D, Molina R, Defilippi BG (2011) Characterization of sugars and organic acids in commercial varieties of table grapes. Chil J Agr Res 71:452–458CrossRefGoogle Scholar
  24. Niesz K, Siska A, Vesselényi I, Hernadi K, Méhn D, Galbács G, Kónya Z, Kiricsi I (2002) Mechanical and chemical breaking of multiwalled carbon nanotubes. Catal Today 76:3–10CrossRefGoogle Scholar
  25. Niu X, Zhao H, Lan M, Zhou L (2015) Platinum nanoparticles encapsulated in carbon microspheres: toward electro-catalyzing glucose with high activity and stability. Electrochim Acta 151:326–331CrossRefGoogle Scholar
  26. Ren J, Shi W, Li K, Ma Z (2012) Ultrasensitive platinum nanocubes enhanced amperometric glucose biosensor based on chitosan and Nafion film. Sensors Actuators B Chem 163:115–120CrossRefGoogle Scholar
  27. Scognamiglio V (2013) Nanotechnology in glucose monitoring: advances and challenges in the last 10 years. Biosens Bioelectron 47:12–25CrossRefGoogle Scholar
  28. Shen Q, Jiang L, Zhang H, Min Q, Hou W, Zhu J-J (2008) Three-dimensional dendritic Pt nanostructures: sonoelectrochemical synthesis and electrochemical applications. J Phys Chem C 112:16385–16392CrossRefGoogle Scholar
  29. Stojanović ZS, Mehmeti E, Kalcher K, Guzsvány V, Stanković DM (2016) SWCNT-modified carbon paste electrode as an electrochemical sensor for histamine determination in alcoholic beverages. Food Anal Methods 9:2701–2710CrossRefGoogle Scholar
  30. Tudorache M, Bala C (2007) Biosensors based on screen-printing technology, and their applications in environmental and food analysis. Anal Bioanal Chem 388:565–578CrossRefGoogle Scholar
  31. Veseli A, Hajrizi A, Arbneshi T, Kalcher K (2012) A new amperometric glucose biosensor based on screen printed carbon electrodes with rhenium (IV)-oxide as mediator. J Electrochem Sci Eng 2:199–210Google Scholar
  32. Viswanathan S, Radecka H, Radecki J (2009) Electrochemical biosensors for food analysis. Monatsh Chem 140:891–899CrossRefGoogle Scholar
  33. Wang J (2001) Glucose biosensors: 40 years of advances and challenges. Electroanalysis 13:983–988CrossRefGoogle Scholar
  34. Wang J (2005) Nanomaterial-based electrochemical biosensors. Analyst 130:421–426CrossRefGoogle Scholar
  35. Wang J (2008) Electrochemical glucose biosensors. Chem Rev 108:814–825CrossRefGoogle Scholar
  36. Wang Y, Yuan R, Chaia Y, Li W, Zhuo Y, Yuan Y, Li J (2011) Direct electron transfer: electrochemical glucose biosensor based on hollow Pt nanosphere functionalized multiwall carbon nanotubes. J Mol Catal B Enzym 71:146–151CrossRefGoogle Scholar
  37. Wu G-H, Song X-H, Wu Y-F, Chen X-M, Luo F, Chen X (2013) Non-enzymatic electrochemical glucose sensor based on platinum nanoflowers supported on graphene oxide. Talanta 105:379–385CrossRefGoogle Scholar
  38. Wu H, Wang J, Kang X, Wang C, Wang D, Liu J, Aksay IA, Lin Y (2009) Glucose biosensor based on immobilization of glucose oxidase in platinum nanoparticles/graphene/chitosan nanocomposite film. Talanta 80:403–406CrossRefGoogle Scholar
  39. Xie J, Wang S, Aryasomayajula L, Varadan VK (2007) Platinum decorated carbon nanotubes for highly sensitive amperometric glucose sensing. Nanotechnology 18:1–9Google Scholar
  40. Yang H, Zhu Y (2007) Glucose biosensor based on nano-SiO2 and “unprotected” Pt nanoclusters. Biosens Bioelectron 22:2989–2993CrossRefGoogle Scholar
  41. Yoo E-H, Lee S-Y (2010) Glucose biosensors: an overview of use in clinical practice. Sensors 10:4558–4576CrossRefGoogle Scholar
  42. Zeng Z, Zhou X, Huang X, Wang Z, Yang Y, Zhang Q, Boey F, Zhang H (2010) Electrochemical deposition of Pt nanoparticles on carbon nanotube patterns for glucose detection. Analyst 135:1726–1730CrossRefGoogle Scholar
  43. Zou Y, Xiang C, Sun L-X, Xu F (2008) Glucose biosensor based on electrodeposition of platinum nanoparticles onto carbon nanotubes and immobilizing enzyme with chitosan-SiO2 sol–gel. Biosens Bioelectron 23:1010–1016CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Valéria Guzsvány
    • 1
    Email author
  • Jasmina Anojčić
    • 1
  • Olga Vajdle
    • 1
  • Emil Radulović
    • 1
  • Dániel Madarász
    • 2
  • Zoltán Kónya
    • 2
    • 3
  • Kurt Kalcher
    • 4
  1. 1.Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental ProtectionUniversity of Novi SadNovi SadSerbia
  2. 2.Department of Applied and Environmental ChemistryUniversity of SzegedSzegedHungary
  3. 3.MTA-SZTE Reaction Kinetics and Surface Chemistry Research GroupSzegedHungary
  4. 4.Institute of Chemistry-Analytical ChemistryKarl-Franzens University GrazGrazAustria

Personalised recommendations