Fast Analysis of Total Polyphenol Content and Antioxidant Activity in Wines and Oenological Tannins Using a Flow Injection System with Tandem Diode Array and Electrochemical Detections
- 56 Downloads
An analytical method for simultaneous determination of total polyphenol content (TPC) and antioxidant activity (AA) of wines (white and red wines) and oenological tannins, using a flow injection system with sequential diode array and electrochemical amperometry detectors (DAD-ECD), was proposed. The signal at 280 nm provided aggregate data for TPC. The anodic peak related to wine phenolic oxidation was scanned using pulsed integrated amperometry over the potential of 800 mV vs. Ag/AgCl, to obtain AA. Serial dilutions avoided the poisoning at the glassy carbon (GC) electrode and the linear response obtained with both detectors was compared with spectrophotometric assays commonly used in oenology laboratory. Intraday and interday analytical repetitions showed a good repeatability and reproducibility (relative standard deviation RSD < 6% for both detectors), and the satisfactory relationship between the proposed coupled flow injection/DAD-ECD and the classic UV methods (R2TPC = 0.9967; R2DPPH = 0.9621) confirmed the efficacy of flow injection analysis with a coupled detection system, for the reliable quality control of wine and wine-related products.
KeywordsTotal polyphenol content Antioxidant activity Electrochemistry Flow injection DPPH• Method comparison
Authors gratefully acknowledge the HTS Enologia and Laffort companies for having supplied food-grade tannin samples.
Authors A.R. and N.T. had PhD grants by the University of Bologna (Italy) and Penta EU program, respectively. Author V.I.P. was funded by the ERASMUS Mundus Action 2 Project while carrying out this experiment.
Compliance with Ethical Standards
Conflict of Interest
Arianna Ricci declares that she has no conflict of interest. Nemanja Teslic declares that he has no conflict of interest. Violeta-Ivanova Petropolus declares that she has no conflict of interest. Giuseppina Paola Parpinello declares that she has no conflict of interest. Andrea Versari declares that she has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Arteaga JF, Ruiz-Montoya M, Palma A, Alonso-Garrido G, Pintado S, Rodríguez-Mellado JM (2012) Comparison of the simple cyclic voltammetry (CV) and DPPH assays for the determination of antioxidant capacity of active principles. Molecules 17:5126–5138. https://doi.org/10.3390/molecules17055126 CrossRefGoogle Scholar
- Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M (2014) Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. J Sci Food Agric 95:204–209. https://doi.org/10.1002/jsfa.6706 CrossRefGoogle Scholar
- Dicu T, Postescu ID, Tatomir C, Tămaş M, Dinu A, Cosma C (2010) A novel method to calculate the antioxidant parameters of the redox reaction between polyphenolic compounds and the stable DPPH radical. Ital J Food Sci 22:330–336Google Scholar
- Harbertson JF, Spayd S (2006) Measuring phenolics in the winery. Am J Enol Vitic 57:280–288Google Scholar
- Haslam E (1998) Practical polyphenolics: from structure to molecular recognition and physiological action. Cambridge University Press, CambridgeGoogle Scholar
- Mannino S, Brenna O, Buratti S, Cosio MS (1998) A new method for the evaluation of the ‘antioxidant power’ of wines. Electroanalysis 10:908–912. https://doi.org/10.1002/(SICI)1521-4109(199810)10:13<908::AID-ELAN908>3.0.CO;2-L CrossRefGoogle Scholar
- Ribéreau-Gayon P (1970) Le dosage des composés phénoliques totaux dans les vins rouges. Chim Anal 52:627–631Google Scholar