Food Analytical Methods

, Volume 12, Issue 1, pp 305–312 | Cite as

A Flow Injection Chemiluminescent Immunosensor for Ultrasensitive Detection of Brombuterol Based on Resin Beads and Enzymatic Amplification

  • Xinchun Zhou
  • Yuting Li
  • Jing Shi
  • Kang Zhao
  • Anping DengEmail author
  • Jianguo LiEmail author


This study described the use of a rapid and sensitive flow injection chemiluminescence immunoassay based on the specific binding of antigens and antibodies for the detection of brombuterol residues in the swine meat and feed samples. Carboxylic resin beads were used as a solid-phase carrier for supporting more coating antigens through acylamide bonds. Under the mechanism of competitive immunity, the brombuterol in solution competed with the coating antigen immobilized on the resin beads for the limited binding sites of antibody. Then, horseradish peroxidase-labeled secondary antibodies were introduced into the immunosensor to combine with polyclonal antibodies. The chemiluminescence intensity of the luminol-p-iodophenol-H2O2 system was catalyzed by horseradish peroxidase, and thus, signal amplification was achieved. Under the optimal conditions of the concentration of coating antigen, antibody and enzymatic secondary antibody, brombuterol could be detected quantitatively. The chemiluminescence intensity decreased linearly with the logarithm of the brombuterol concentration in the range of 0.001 to 300 ng mL−1. The limit of detection (3σ) and the limit of quantitation were 0.33 pg mL−1 and 1.1 pg mL−1, respectively. This method had undergone a series of test conditions and exhibited good specificity, stability, and reproducibility. The immunosensor can also detect real samples and provide prospects for the detection of other small molecule compounds, such as isoproterenol, enrofloxacin, and ofloxacin.


Chemiluminescence immunoassay Carboxylic resin beads Signal amplification Luminol-p-iodophenol-H2O2 Brombuterol 


Funding information

This work was financially supported by the Science Fund from the National Natural Science Foundation of China (no.21175097, no.31772053), the Project of State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1716), the Suzhou Industry, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials (SYG201636), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (no.YX10900212).

Compliance with Ethical Standards

Conflict of Interest

The authors have read and complied with the statement of ethical standards for manuscripts submitted to Food Analytical Methods. All the authors listed have approved the submission: Xinchun Zhou declares that she has no conflict of interest. Yuting Li declares that she has no conflict of interest. Jing Shi declares that she has no conflict of interest. Kang Zhao declares that she has no conflict of interest. Anping Deng declares that he has no conflict of interest. Jianguo Li declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human subject. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials ScienceSoochow UniversitySuzhouChina

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