Advertisement

Food Analytical Methods

, Volume 12, Issue 1, pp 229–238 | Cite as

Detection of Phosphatidylcholine Content in Crude Oil with Bio-Enzyme Screen-Printed Electrode

  • Dianyu Yu
  • Dezhi Zou
  • Dan Li
  • Xu Wang
  • Xin Zhang
  • Changhua Yu
  • Liqi WangEmail author
  • Walid ElfallehEmail author
  • Lianzhou JiangEmail author
Article
  • 34 Downloads

Abstract

A low-cost bio-enzyme screen-printed electrode (SPE) was produced by immobilizing choline oxidase (ChOx) and horseradish peroxidase (HRP) on an SPE with MWCNTs/SnO2/CS as modified material. The conditions of ChOx were as follows: concentration of 3 U, HRP concentration of 4 U, and pH 7.5. The bio-enzyme SPE had a significant electrochemical response to phosphatidylcholine (PC); the linear relationship between the peak current and PC content ranged from 30 to 270 mg/L, and the detection limit was 3 mg/L (S/N = 3). Bio-enzyme SPE was used to detect the content of PC in soybean crude oil. The spiked recovery of the samples ranged from 96.68 to 106.21%. The detection results obtained using high-performance liquid chromatography (HPLC) confirmed the results of the bio-enzyme SPE with a high correlation (r2 = 0.9978). After 45 days of storage of the bio-enzyme SPE, the current value remained 90% of initial current. The reproducibility and stability of the PC content detected by the bio-enzyme SPE in soybean crude oil exhibited a high performance.

Keywords

Choline oxidase Horseradish peroxidase Screen-printed electrode Soybean crude oil Phosphatidylcholine content Rapid detection 

Notes

Author Contributions

Dezhi Zou, Dan Li, Xu Wang, Xin Zhang Changhua Yu, and Walid Elfalleh conceived and designed the experiments. Dianyu Yu, Dezhi Zou, and Lianzhou Jiang performed the experiments. Dezhi Zou and Changhua Yu analyzed the data (designed figures and tables). Dan Li, Xu Wang, and Xin Zhang contributed in reagents, materials, and analysis tools. Dianyu Yu, Dezhi Zou Dan Li, and Liqi Wang wrote the paper. Walid Elfalleh corrected and submitted the paper.

Funding Information

This work was supported by a grant from the National Natural Science Foundation of China (NSFC): Study on the mechanism of nanomagnetic enzyme hydrolysis of soybean oil by multi-effect orientation and biosynthesis of functional lipids (No. 31571880). This work was supported by a grant from the Province Natural Science Foundation of Heilongjiang: Study on the Mechanism of Continuous Orientation Esterification of Nanometer Magnetic Lipase (No. C2017019). This work was also supported by a grant from the National Research Project in 13th Five-Year: Research on Key Technologies of green soybean oil production and large scale intelligent equipment. (No. 2016YFD0401402). This work was also supported by a grant from the National Research Project in 13th Five-Year: Rice bran high value steady state processing technology and intelligent equipment development and demonstration. (No. 2018YFD0401101).

Compliance with Ethical Standards

Conflict of Interest

Dianyu Yu declares that he has no conflict of interest. Dezhi Zou declares that he has no conflict of interest. Dan Li declares that he has no conflict of interest. Xu Wang declares that he has no conflict of interest. Xin Zhang declares that he has no conflict of interest. Changhua Yu declares that he has no conflict of interest. Liqi Wang declares that he has no conflict of interest. Walid Elfalleh declares that he has no conflict of interest. Lianzhou Jiang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

References

  1. Baskeyfield DE, Davis F, Magan N, Tothill IE (2011) A membrane-based immunosensor for the analysis of the herbicide isoproturon. Anal Chim Acta 699:223–231CrossRefGoogle Scholar
  2. Cai X, Gao X, Wang L, Wu Q, Lin X (2013) A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection. Sensors Actuators B Chem 181:575–583CrossRefGoogle Scholar
  3. De Paz P, Esteso MC, Alvarez M et al (2010) Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology 74:663–671CrossRefGoogle Scholar
  4. Deng C, Chen J, Nie Z, Si S (2010) A sensitive and stable biosensor based on the direct electrochemistry of glucose oxidase assembled layer-by-layer at the multiwall carbon nanotube-modified electrode. Biosens Bioelectron 26:213–219CrossRefGoogle Scholar
  5. Dhand C, Singh SP, Arya SK, Datta M, Malhotra BD (2007) Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension. Anal Chim Acta 602:244–251CrossRefGoogle Scholar
  6. Di Fusco M, Tortolini C, Deriu D, Mazzei F (2010) Laccase-based biosensor for the determination of polyphenol index in wine. Talanta 81:235–240CrossRefGoogle Scholar
  7. Donato P, Cacciola F, Cichello F, Russo M, Dugo P, Mondello L (2011) Determination of phospholipids in milk samples by means of hydrophilic interaction liquid chromatography coupled to evaporative light scattering and mass spectrometry detection. J Chromatogr A 1218:6476–6482CrossRefGoogle Scholar
  8. Feng S, Cai Z, Zuo R, Mai K, Ai Q (2017) Effects of dietary phospholipids on growth performance and expression of key genes involved in phosphatidylcholine metabolism in larval and juvenile large yellow croaker, Larimichthys crocea. Aquaculture 469:59–66CrossRefGoogle Scholar
  9. Haghighi B, Tabrizi MA (2013) Direct electron transfer from glucose oxidase immobilized on an overoxidized polypyrrole film decorated with Au nanoparticles. Colloids Surf B Biointerfaces 103:566–571CrossRefGoogle Scholar
  10. Hernández-Ibáñez N, García-Cruz L, Montiel V, Foster CW, Banks CE, Iniesta J (2016) Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures. Biosens Bioelectron 77:1168–1174CrossRefGoogle Scholar
  11. Ibrahim H, Caudron E, Kasselouri A, Prognon P (2010) Interest of fluorescence derivatization and fluorescence probe assisted post-column detection of phospholipids: a short review. Molecules 15:352–373CrossRefGoogle Scholar
  12. Li Y, Wang F, Huang F, Li Y, Feng S (2012) Direct electrochemistry of glucose oxidase and its biosensing to glucose based on the Chit-MWCNTs–AuNRs modified gold electrode. J Electroanal Chem 685:86–90CrossRefGoogle Scholar
  13. Liu B, Cao Y, Chen D, Kong J, Deng J (2003) Amperometric biosensor based on a nanoporous ZrO2 matrix. Anal Chim Acta 478:59–66CrossRefGoogle Scholar
  14. MacKenzie A, Vyssotski M, Nekrasov E (2009) Quantitative analysis of dairy phospholipids by 31 P NMR. J Am Oil Chem Soc 86:757–763CrossRefGoogle Scholar
  15. Martinez AI, Acosta DR (2005) Effect of the fluorine content on the structural and electrical properties of SnO2 and ZnO–SnO2 thin films prepared by spray pyrolysis. Thin Solid Films 483:107–113CrossRefGoogle Scholar
  16. Mitchell KM (2004) Acetylcholine and choline amperometric enzyme sensors characterized in vitro and in vivo. Anal Chem 76:1098–1106CrossRefGoogle Scholar
  17. More NS, Gogate PR (2018a) Intensified degumming of crude soybean oil using cavitational reactors. J Food Eng 218:33–43CrossRefGoogle Scholar
  18. More NS, Gogate PR (2018b) Ultrasound assisted enzymatic degumming of crude soybean oil. Ultrason Sonochem 42:805–813CrossRefGoogle Scholar
  19. Nangia Y, Bhalla V, Kumar B, Suri CR (2012) Electrochemical stripping voltammetry of gold ions for development of ultra-sensitive immunoassay for chlorsulfuron. Electrochem Commun 14:51–54CrossRefGoogle Scholar
  20. Rahman MM, Ahammad AJ, Jin J-H et al (2010) A comprehensive review of glucose biosensors based on nanostructured metal-oxides. Sensors 10:4855–4886CrossRefGoogle Scholar
  21. Rahman M, Li X, Jeon Y-D et al (2012) Simultaneous determination of ranitidine and metronidazole at poly (thionine) modified anodized glassy carbon electrode. J Electrochem Sci Technol 3:90–94CrossRefGoogle Scholar
  22. Rahman MM, Li X, Kim J, Lim BO, Ahammad AJS, Lee JJ (2014) A cholesterol biosensor based on a bi-enzyme immobilized on conducting poly (thionine) film. Sens Actuators B Chem 202:536–542CrossRefGoogle Scholar
  23. Razola SS, Pochet S, Grosfils K, Kauffmann JM (2003) Amperometric determination of choline released from rat submandibular gland acinar cells using a choline oxidase biosensor. Biosens Bioelectron 18:185–191CrossRefGoogle Scholar
  24. Renedo OD, Alonso-Lomillo MA, Martínez MA (2007) Recent developments in the field of screen-printed electrodes and their related applications. Talanta 73:202–219CrossRefGoogle Scholar
  25. Safina G, Ludwig R, Gorton L (2010) A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes. Electrochim Acta 55:7690–7695CrossRefGoogle Scholar
  26. Senthilkumar V, Vickraman P, Jayachandran M, Sanjeeviraja C (2010) Synthesis and characterization of SnO2 nanopowder prepared by precipitation method. J Dispers Sci Technol 31:1178–1181CrossRefGoogle Scholar
  27. Varma S (2002) Electrochemical studies on reconstituted horseradish peroxidase modified carbon paste electrodes. Bioelectrochemistry 56:107–111CrossRefGoogle Scholar
  28. Véronique Gibon AT (2000) Removal of gums and waxes: a review. Inform 11:524–535Google Scholar
  29. Wang J, Musameh M, Lin Y (2003) Solubilization of carbon nanotubes by Nafion toward the preparation of amperometric biosensors. J Am Chem Soc 125:2408–2409CrossRefGoogle Scholar
  30. Wei X, Cruz J, Gorski W (2002) Integration of enzymes and electrodes: spectroscopic and electrochemical studies of chitosan- enzyme films. Anal Chem 74:5039–5046CrossRefGoogle Scholar
  31. Yang M, Yang Y, Yang Y, Shen G, Yu R (2004) Bienzymatic amperometric biosensor for choline based on mediator thionine in situ electropolymerized within a carbon paste electrode. Anal Biochem 334:127–134CrossRefGoogle Scholar
  32. Yu D, Ma Y, Xue SJ, Jiang L, Shi J (2013) Characterization of immobilized phospholipase A1 on magnetic nanoparticles for oil degumming application. LWT-Food Sci Technol 50:519–525CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Food ScienceNortheast Agricultural UniversityHarbinChina
  2. 2.School of Computer and Information EngineeringHarbin University of CommerceHarbinChina
  3. 3.UR Catalyse et Matériaux pour l’Environnement et les Procédés URCMEP (UR11ES85), Faculté des Sciences de GabèsUniversité de GabèsGabèsTunisia

Personalised recommendations