Food Analytical Methods

, Volume 12, Issue 1, pp 166–175 | Cite as

Determination of Biogenic Amines in Fish Meat and Fermented Foods Using Column-Switching High-Performance Liquid Chromatography with Fluorescence Detection

  • Mami Ishimaru
  • Yuma Muto
  • Akari Nakayama
  • Hideo Hatate
  • Ryusuke TanakaEmail author


In this study, we developed a column-switching high-performance liquid chromatography method with fluorescence detection for the simultaneous analysis of biogenic amines (BAs). This method uses an isocratic solution using acetonitrile with water as the mobile phase. Column-switching is achieved by using a switching valve with a set time program to change flow direction. Using this method, seven BAs (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, and spermidine) could be separated and impurities eliminated. Using fluorescence detection, BAs could be identified with high sensitivity. We employed this method to determine the BA contents of fish (mackerel, tuna, and cod) and fermented food (a soybean product and cheese). Our results are in line with previous reports, yielded highly reproducible and quantitative results, and enabled the quick and simultaneous analysis of multiple BAs. Therefore, the method developed here may be useful for the continuous analysis of BAs in different samples to evaluate food quality.


Biogenic amines Column-switching HPLC Fluorescence detection Fish Fermented food 


Funding Information

This work was supported by the Grant-in-Aid for JSPS Research Fellow Grant Number 17J01852.

Compliance with Ethical Standards

Conflict of Interest

Mami Ishimaru declares that she has no conflict of interest. Yuma Muto declares that he has no conflict of interest. Akari Nakayama declares that she has no conflict of interest. Hideo Hatate declares that he has no conflict of interest. Ryusuke Tanaka declares that he has no conflict of interest.

Ethical Approval

Not applicable.

Informed Consent

Not applicable.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Applied Biological Science, Interdisciplinary Graduate School of Agriculture and EngineeringUniversity of MiyazakiMiyazakiJapan
  2. 2.Department of Marine Biology and Environmental Sciences, Faculty of AgricultureUniversity of MiyazakiMiyazakiJapan

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