Food Analytical Methods

, Volume 12, Issue 4, pp 877–886 | Cite as

Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk

  • Jianwen Qu
  • Huijuan Xie
  • Shuying Zhang
  • Pengjie Luo
  • Ping Guo
  • Xianxiong Chen
  • Yuebin Ke
  • Junyu Zhuang
  • Fangmei Zhou
  • Wenxiao JiangEmail author


Microsphere-based immunoassays involving flow cytometry have recently gained popularity for use in protein detection and infectious disease diagnosis due to their simple assay format and capacity for multiplexed analysis. In this work, four mycotoxins, including aflatoxin M1 (AFM1), ochratoxin A (OTA), deoxynivalenol (DON), and fumonisin B1 (FB1), were selected as model analytes. A quadplex flow cytometric immunoassay (FCIA) was developed for detecting multiple mycotoxin residues in milk. The optimized quadplex FCIA exhibited satisfactory compatibility with the multiple mycotoxin residue analysis, with limits of detection (LODs) of 0.045 μg L−1 for AFM1, 0.94 μg L−1 for OTA, 7.48 μg L−1 for DON, and 2.45 μg L−1 for FB1. The recoveries of the target mycotoxins from spiked milk were 76–95%, with a relative standard deviation of less than 13.4%. Compared to traditional ELISA formats, the FCIA provided lower detection limits for multiple mycotoxin detection.


Mycotoxin Multi-analyte residue Flow cytometric immunoassay Milk Food safety 


Funding Information

This work was supported by the National Natural Science Foundation of China (No. 31602103); the Science and Technology Planning Project of Guangdong Province, China (Nos. 2016A020210055 and 2017B020207009); the Shenzhen Basic Research Project (Nos. JCYJ20160307114724751 and JCYJ20170817095240632); and the Shenzhen Sanming Project of Medicine and Health (No. SZSM201611068).

Compliance with Ethical Standards

Conflict of Interest

Jianwen Qu declares that he has no conflict of interest. Huijuan Xie declares that he has no conflict of interest. Shuying Zhang declares that he has no conflict of interest. Pengjie Luo declares that he has no conflict of interest. Ping Guo declares that he has no conflict of interest. Yuebin Ke declares that he has no conflict of interest. Junyu Zhuang declares that he has no conflict of interest. Fangmei Zhou declares that he has no conflict of interest. Wenxiao Jiang declares that he has no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed Consent

Not applicable.

Supplementary material

12161_2018_1412_MOESM1_ESM.docx (21 kb)
ESM 1 (DOCX 20 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Physiology, School of Basic Medical SciencesShenzhen University Health Sciences CenterShenzhenChina
  2. 2.Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk AssessmentMinistry of HealthBeijingChina
  3. 3.Jiangxi Food Inspection and Testing Research InstituteNanchangChina
  4. 4.Key Laboratory of Molecular Epidemiology of ShenzhenShenzhen Center for Disease Control and PreventionShenzhenChina
  5. 5.Guangdong Food Quality Supervision and Inspection StationGuangdong Food Industry InstituteGuangzhouChina

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