Extensive Validation of Gas Chromatographic Method for Determination of Esters in Wines Using Monolithic Materials as Preconcentration Step
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A comprehensive validation of a quantitative method is a critical issue for food analysis, which requires reliable estimation of uncertainties influencing measurements. Esters as important contributors to fruity aroma generally affect quality of wine and its organoleptic features. In this study, a full validation of the method developed for quantification of esters with uncommon silica monolithic discs and gas chromatographic analysis is presented. Two types of sorbent modification and a few desorption solvents were tested for ester removal from wine samples. Uncertainty contributions from mass balance tolerance, purity of chemicals, volume measurements, calibration curve deviation and extraction procedure were included in the calculation of the combined uncertainty. The expanded uncertainty of ester determination ranged from 15 to 37% and was mainly affected by uncertainty of calibration curve. The proposed method provided a limit of detection and a limit of quantification of 0.005–0.168 μg/L and 0.018–0.560 μg/L, respectively. Additional measurements revealed a recovery range of 70–111%, average reproducibility of instrument response less than 4%, and repeatability of the method below 16% for most of esters.
KeywordsValidation Esters Monolithic material Wine GC-FID
This work was supported under kind support of Slovak Research and Development Agency under contract number APVV-15-0333
Research Involving Human Participants and/or Animals or Ethical Approval
This article does not contain studies with human participants or animals performed by any of the authors.
Compliance with Ethical Standards
Conflict of Interest
Olga Vyviurska declares that she has no conflict of interest. Roman Gorovenko declares that he has no conflict of interest. Veronika Panáková declares that she has no conflict of interest. Ivan Špánik declares that he has no conflict of interest.
- Antalick G, Suklje K, Blackman JW, Meeks C, Deloire A, Schmidtke LM (2015) Influence of grape composition on red wine ester profile: comparison between Cabernet Sauvignon and Shiraz cultivars from Australian warm climate. J Agric Food Chem 63:4664–4672. https://doi.org/10.1021/acs.jafc.5b00966 CrossRefGoogle Scholar
- Arabi M, Ghaedi M, Ostovan A (2017) Synthesis and application of in-situ molecularly imprinted silica monolithic in pipette-tip solid-phase microextraction for the separation and determination of gallic acid in orange juice samples. J Chromatogr B Anal Technol Biomed Life Sci 1048:102–110. https://doi.org/10.1016/j.jchromb.2017.02.016 CrossRefGoogle Scholar
- Azzolini M, Tosi E, Lorenzini M, Finato F, Zapparoli G (2015) Contribution to the aroma of white wines by controlled Torulaspora delbrueckii cultures in association with Saccharomyces cerevisiae. World J Microbiol Biotechnol 31:277–293. https://doi.org/10.1007/s11274-014-1774-1 CrossRefGoogle Scholar
- Eurachem/CITAC guide: quantifying uncertainty in analytical measurement (2012). Third edition edn.,Google Scholar
- Ferreira V, Lopez R, Cacho JF (2000) Quantitative determination of the odorants of young red wines from different grape varieties. J Sci Food Agric 80:1659–1667. https://doi.org/10.1002/1097-0010(20000901)80:11<1659::Aid-Jsfa693>3.0.Co;2-6 CrossRefGoogle Scholar
- Grainger K (2009) Wine quality: tasting and selection. Wine quality: Tasting and Selection https://doi.org/10.1002/9781444301687
- Insuan W, Khawmodjod P, Whitlow HJ, Soonthondecha P, Malem F, Chienthavorn O (2016) High-throughput and low-cost analysis of trace volatile phthalates in seafood by online coupling of monolithic capillary adsorbent with GC-MS. J Agric Food Chem 64:3287–3292. https://doi.org/10.1021/acs.jafc.6b00742 CrossRefGoogle Scholar
- Liu H et al (2016a) Novel method for the rapid and specific extraction of multiple beta2 -agonist residues in food by tailor-made monolith-MIPs extraction disks and detection by gas chromatography with mass spectrometry. J Sep Sci 39:3578–3585. https://doi.org/10.1002/jssc.201600479 CrossRefGoogle Scholar
- Monolithic Silica Adsorbents MonoTrapTM. (2014) GL Sciences Inc. . https://www.glsciences.com/wp/wp-content/uploads/2013/06/MonoTrap20140218KH.pdf. Accessed 23.7.2018
- OIV-OENO-553-2016 (n.d.) Analysis of volatile compounds in wines by gas chromatography The International Organisation of Vine and Wine 1-10Google Scholar
- Padilla B, Gil J, Manzanares P (2016) Past and future of non-saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.00411
- Schvarczova E, Stefanikova J, Jankura E, Kolek E (2017) Selection of autochthonous Saccharomyces cerevisiae strains for production of typical Pinot Gris wines. J Food Nutr Res 56:389–397Google Scholar
- Seguinot P, Rollero S, Sanchez I, Sablayrolles J-M, Ortiz-Julien A, Camarasa C, Mouret J-R (2018) Impact of the timing and the nature of nitrogen additions on the production kinetics of fermentative aromas by Saccharomyces cerevisiae during winemaking fermentation in synthetic media. Food Microbiol 76:29–39. https://doi.org/10.1016/j.fm.2018.04.005 CrossRefGoogle Scholar