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Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa

  • Yan Li
  • Wenhe Zhong
  • Ann Zhufang Koay
  • Hui Qi Ng
  • Qianhui Nah
  • Yee Hwa Wong
  • Jeffrey Hill
  • Julien LescarEmail author
  • Peter C. DedonEmail author
  • CongBao KangEmail author
Article

Abstract

Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation, making it an important target for the development of new antibacterial compounds. TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-l-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer. Here we report the backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa. Most resonances were assigned and the secondary structure for each amino acid was determined according to the assigned backbone resonances. The availability of the assignment will be valuable for exploring molecular interactions of TrmD with ligands, inhibitors and tRNA.

Keywords

TrmD Pseudomonas aeruginosa tRNA methyltransferase Epitranscriptome Drug discovery Antibacterial Protein dynamics Backbone assignment 

Notes

Acknowledgements

CK appreciates the support from NMRC OF-IRG Grant (NMRC/OFIRG/0051/2017) and A*STAR JCO Grant (1431AFG102/1331A028). This work is also supported by National Research Foundation of Singapore through the Singapore-MIT-Alliance for Research and Technology (SMART) Infectious Disease and Antimicrobial Resistance Interdisciplinary Research Groups. WZ was supported by a SMART Scholar Fellowship. We also thank Prof Ho Sup Yoon and Dr. Hong Ye from Nanyang Technological University for the NMR experiments. The authors appreciate the valuable discussion from the team members at EDDC, A*STAR.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Experimental Drug Development CentreSingaporeSingapore
  2. 2.Infectious Disease and Antimicrobial Resistance Interdisciplinary Research GroupsSingapore-MIT Alliance for Research and TechnologySingaporeSingapore
  3. 3.NTU Institute of Structural BiologyNanyang Technological UniversitySingaporeSingapore
  4. 4.School of Biological SciencesNanyang Technological UniversitySingaporeSingapore
  5. 5.Department of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  6. 6.Department of Pathogen Biology, School of Basic Medicine, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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