Biomolecular NMR Assignments

, Volume 12, Issue 2, pp 351–355 | Cite as

Backbone and side chain NMR assignments for the ribosome Elongation Factor P (EF-P) from Staphylococcus aureus

  • Konstantin S. UsachevEmail author
  • Alexander A. Golubev
  • Shamil Z. Validov
  • Vladimir V. Klochkov
  • Albert V. Aganov
  • Iskander Sh. Khusainov
  • Marat M. Yusupov


Elongation Factor P (EF-P) is a 20.5 kDa protein that provides specialized translation of special stalling amino acid motifs. Proteins with stalling motifs are often involved in various processes, including stress resistance and virulence. Thus it has been shown that the virulent properties of microorganisms can be significantly reduced if the work of EF-P is disrupted. In order to elucidate the structure, dynamics and function of EF-P from Staphylococcus aureus (S. aureus), here we report backbone and side chains 1H, 13C and 15N chemical shift assignments of EF-P. Analysis of the backbone chemical shifts by TALOS+ suggests that EF-P contains 1 α-helix and 13 β-strands (β1-β2-β3-β4-β5-β6-β7-α1-β8-β9-β10-β11-β12-β13). The solution of the structure of this protein by NMR and X-ray diffraction analysis, as well as the structure of the ribosome complex by cryo-electron microscopy, will allow further screening of highly selective inhibitors of the translation of the pathogenic bacterium S. aureus. Here we report the almost complete 1H, 13C, 15N backbone and side chain NMR assignment of a 20.5 kDa EF-P.


EF-P Staphylococcus aureus Ribosome Protein NMR Resonance assignment 


S. aureus

Staphylococcus aureus


Elongation Factor P


Cryo-electron microscopy


Size-exclusion chromatography


Immobilized metal affinity chromatography



This work was supported by the Russian Science Foundation (Grant 17-74-20009).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Konstantin S. Usachev
    • 1
    • 2
    Email author
  • Alexander A. Golubev
    • 1
  • Shamil Z. Validov
    • 1
  • Vladimir V. Klochkov
    • 2
  • Albert V. Aganov
    • 2
  • Iskander Sh. Khusainov
    • 1
    • 3
  • Marat M. Yusupov
    • 1
    • 3
  1. 1.Laboratory of Structural Biology, Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussia
  2. 2.NMR Laboratory, Medical Physics Department, Institute of PhysicsKazan Federal UniversityKazanRussia
  3. 3.Département de Biologie et de Génomique Structurales, Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS UMR7104, INSERM U964Université de StrasbourgIllkirchFrance

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