Chemical shift assignments of RHE_RS02845, a NTF2-like domain-containing protein from Rhizobium etli

  • Tao Li
  • Shuangli Li
  • Chunjie Liang
  • Jiang Zhu
  • Maili Liu
  • Yunhuang Yang
Article
  • 19 Downloads

Abstract

The nuclear transport factor 2 (NTF2) like superfamily includes members of the NTF2 family, delta-5-3-ketosteroid isomerases, and the beta subunit of ring hydroxygenases. This family plays important roles in both eukaryotic and prokaryotic cells, and is taken as a classic example of divergent evolution because proteins in this family exhibit diverse biological functions, although share common structural features. We cloned the gene RHE_RS02845 encoding a predicted NTF2-like domain-containing protein in Rhizobium etli, and prepared U-13C/15N-labeled protein samples for its three-dimensional NMR structural determination. Here, chemical shift assignments for both backbone and side-chain atoms are reported, which is prerequisite for further structural calculation and functional research using NMR spectroscopy.

Keywords

RHE_RS02845 NTF2-like domain Rhizobium etli CFN42 Chemical shift assignments 

Notes

Acknowledgements

This work was supported by funds from the National Natural Sciences Foundation of China (Grant Number: 21575155) and the Hundred Talent Program by Chinese Academy of Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Tao Li
    • 1
    • 2
  • Shuangli Li
    • 1
    • 2
  • Chunjie Liang
    • 1
    • 2
  • Jiang Zhu
    • 1
  • Maili Liu
    • 1
  • Yunhuang Yang
    • 1
  1. 1.State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and MathematicsChinese Academy of SciencesWuhanChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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