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Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820

  • Sanath Kondaveeti
  • Sanjay K. S. Patel
  • Janghun Woo
  • Ji Hyang Wee
  • Sang-Yong Kim
  • Riyadh I. Al-Raoush
  • In-Won KimEmail author
  • Vipin Chandra KaliaEmail author
  • Jung-Kul LeeEmail author
Original research article
  • 45 Downloads

Abstract

A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (Vmax = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity.

Keywords

Schizophyllum commune Cellobiohydrolase Catalytic efficiency Enzyme purification Enzyme production 

Notes

Acknowledgements

This research was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2019R1C1C1009766, NRF-2019H1D3A2A01060226, 2019R1F1A1063131). This research was supported by 2018 KU Brain Pool fellowship of Konkuk University.

Supplementary material

12088_2019_843_MOESM1_ESM.docx (133 kb)
Supplementary material 1 (DOCX 133 kb)

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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of Chemical EngineeringKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Food Science and BiotechnologyShin-Ansan UniversityAnsanRepublic of Korea
  3. 3.Department of Civil and Architectural Engineering, College of EngineeringQatar UniversityDohaQatar

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