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Phomopsis tersa as Inhibitor of Quorum Sensing System and Biofilm Forming Ability of Pseudomonas aeruginosa

  • Himani Meena
  • Rashmi Mishra
  • Sampathkumar Ranganathan
  • V. Venkateswara Sarma
  • Dinakara Rao Ampasala
  • Vipin Chandra KaliaEmail author
  • Jung-Kul LeeEmail author
  • Busi SiddhardhaEmail author
Original research article
  • 74 Downloads

Abstract

Endophytic fungi provide rich reservoir for novel antimicrobial compounds. An endophytic fungus, from Carica papaya plant identified as Phomopsis tersa, was investigated for attenuating the quorum sensing mediated pathogenicity of Pseudomonas aeruginosa PAO1. Crude extract of P. tersa was found to reduce the production of redox-active pigments—pyocyanin and pyoverdine in P. aeruginosa PAO1 by 92.46% and 71.55%, respectively at sub-MIC concentration of 900 μg/mL. In addition, the crude extract was also able to inhibit the expression of virulence factors involved in biofilm formation: exopolysaccharide (72.21%) and alginate (72.50%). Secretion of cell-lytic enzymes was also found to be reduced: chitinase by 79.73% and elastase by 74.30%. 3-Isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione identified from GC-MS analysis, displayed favorable molecular interactions with P. aeruginosa transcriptional regulators, LasR and RhlR with good docking scores of − 6.873 kJ/mol and − 6.257 kJ/mol, respectively. The study thus reveals the potential use of P. tersa for discovering drugs against infectious pathogens.

Keywords

Pseudomonas aeruginosa Quorum sensing Phomopsis tersa Biofilm formation Secondary metabolites 

Notes

Acknowledgements

We thank Fungal Biotechnology Laboratory, Department of Biotechnology, Pondicherry University for isolating the fungi from Carica papaya. Dr. Busi Siddhardha is thankful to the start-up research grant from the Department of Science and Technology – The Science & Engineering Research Board [SB/YS/LS-32/2014] for funding the project work. This work was supported by Brain Pool Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2019H1D3A2A01060226) to work at Konkuk University (VCK). This research was supported by Basic Science Research Program through the NRF funded by the Ministry of Science, ICT & Future Planning (2013M3A6A8073184) (JKL).

Compliance with Ethical Standards

Conflict of interest

The authors hereby declare no conflict of interest.

Supplementary material

12088_2019_840_MOESM1_ESM.doc (48 kb)
Supplementary material 1 (DOC 85 kb)

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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of Microbiology, School of Life SciencesPondicherry UniversityPuducherryIndia
  2. 2.Fungal Biotechnology Lab, Department of Biotechnology, School of Life SciencesPondicherry UniversityPuducherryIndia
  3. 3.Centre for Bioinformatics, School of Life SciencesPondicherry UniversityPuducherryIndia
  4. 4.Department of Chemical EngineeringKonkuk UniversitySeoulRepublic of Korea

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