Indian Journal of Microbiology

, Volume 59, Issue 4, pp 476–481 | Cite as

Exploitation of Citrus Peel Extract as a Feedstock for Power Generation in Microbial Fuel Cell (MFC)

  • Sanath Kondaveeti
  • Gunda Mohanakrishna
  • Anurag Kumar
  • Chunfen Lai
  • Jung-Kul LeeEmail author
  • Vipin C. KaliaEmail author
Original Research article


Microbial fuel cells (MFCs) are envisioned as an evolving cost-effective process for treating organic wastes to simultaneously generate bioelectricity. Therefore, in present study a single chambered mediator- less air cathode MFC was operated for bioelectricity generation using citrus waste (CW) as a feedstock. The MFC was operated at four organic loading conditions (OLs; 3, 6, 9 and 12 kg/m3). The voltage generation and organic content reduction demonstrated the possibility of utilizing CW as a substrate in MFC. The polarization analysis revealed a high-power generation of 71.1 mW/m2 with low OL of 3 kg/m3. The decrease in pH and high volatile fatty acids (VFAs) generation was noted at high OL. Our current findings suggest better performance of MFC, in terms of energy generation and organic reduction at high OL.


Microbial fuel cells Citrus waste Chemical oxygen demand (COD) Power generation Bioelectricity 



This research was supported by Brain Pool Grant (NRF-2019H1D3A2A01060226) by National Research Foundation of Korea to work at Konkuk University (VCK). This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013M3A6A8073184). This research was supported by 2018 KU Brain Pool of Konkuk University.

Supplementary material

12088_2019_829_MOESM1_ESM.docx (157 kb)
Supplementary material 1 (DOCX 156 kb)


  1. 1.
    Kondaveeti S, Abu-Reesh IM, Mohanakrishna G, Pant D, He Z (2019) Utilization of residual organics of Labaneh whey for renewable energy generation through bioelectrochemical processes: strategies for enhanced substrate conversion and energy generation. Bioresour Technol 286:121409. CrossRefPubMedGoogle Scholar
  2. 2.
    Kondaveeti S, Mohanakrishna G, Lee J-K, Kalia VC (2019) Methane as a substrate for energy generation using microbial fuel cells. Indian J Microbiol 59:121–124. CrossRefPubMedGoogle Scholar
  3. 3.
    Singh RK, Singh R, Sivakumar D, Kondaveeti S, Kim T, Li J, Sung BH, Cho B-K, Kim DR, Kim SC, Kalia VC, Zhang Y-HPJ, Zhao H, Kang YC, Lee J-K (2018) Insights into cell-free conversion of CO2 to chemicals by a multienzyme cascade reaction. ACS Catal 8:11085–11093. CrossRefGoogle Scholar
  4. 4.
    Kondaveeti SK, Seelam JS, Mohanakrishna G (2018) Anodic electron transfer mechanism in bioelectrochemical systems. In: Das D (ed) Microbial fuel cell: a bioelectrochemical system that converts waste to watts. Springer, Cham, pp 87–100. CrossRefGoogle Scholar
  5. 5.
    Kondaveeti S, Mohanakrishna G, Pagolu R, Kim I-W, Kalia VC, Lee J-K (2019) Bioelectrogenesis from raw algal biomass through microbial fuel cells: effect of acetate as co-substrate. Indian J Microbiol 59:22–26. CrossRefPubMedGoogle Scholar
  6. 6.
    Mohanakrishna G, Abu-Reesh IM, Kondaveeti S, Al-Raoush RI, He Z (2018) Enhanced treatment of petroleum refinery wastewater by short-term applied voltage in single chamber microbial fuel cell. Bioresour Technol 253:16–21. CrossRefPubMedGoogle Scholar
  7. 7.
    Venkata Mohan S, Mohanakrishna G, Reddy BP, Saravanan R, Sarma PN (2008) Bioelectricity generation from chemical wastewater treatment in mediatorless (anode) microbial fuel cell (MFC) using selectively enriched hydrogen producing mixed culture under acidophilic microenvironment. Biochem Engn J 39:121–130. CrossRefGoogle Scholar
  8. 8.
    Chae K-J, Choi M-J, Lee J-W, Kim K-Y, Kim IS (2009) Effect of different substrates on the performance, bacterial diversity, and bacterial viability in microbial fuel cells. Bioresour Technol 100:3518–3525. CrossRefPubMedGoogle Scholar
  9. 9.
    Mohanakrishna G, Venkata Mohan S, Sarma PN (2010) Utilizing acid-rich effluents of fermentative hydrogen production process as substrate for harnessing bioelectricity: an integrative approach. Int J Hydrog Energy 35:3440–3449. CrossRefGoogle Scholar
  10. 10.
    Sharma K, Mahato N, Cho MH, Lee YR (2017) Converting citrus wastes into value-added products: economic and environmently friendly approaches. Nutrition 34:29–46. CrossRefPubMedGoogle Scholar
  11. 11.
    Marín FR, Soler-Rivas C, Benavente-García O, Castillo J, Pérez-Alvarez JA (2007) By-products from different citrus processes as a source of customized functional fibres. Food Chem 100:736–741. CrossRefGoogle Scholar
  12. 12.
    Crawshaw R (2003) Co-product feeds: animal feeds from the food and drinks industries. R Crawshaw Nottingham University Press, Nottingham, 2001 pp 285. J Sci Food Agr 83:362–362. CrossRefGoogle Scholar
  13. 13.
    Venkata Mohan S, Lenin Babu M, Venkateswar Reddy M, Mohanakrishna G, Sarma PN (2009) Harnessing of biohydrogen by acidogenic fermentation of citrus limetta peelings: effect of extraction procedure and pretreatment of biocatalyst. Int J Hydrog Energy 34:6149–6156. CrossRefGoogle Scholar
  14. 14.
    Khan AM, Obaid M (2015) Comparative bioelectricity generation from waste citrus fruit using a galvanic cell, fuel cell and microbial fuel cell. J Energy South Afr 26:90–99CrossRefGoogle Scholar
  15. 15.
    Kakarla R, Min B (2014) Photoautotrophic microalgae Scenedesmus obliquus attached on a cathode as oxygen producers for microbial fuel cell (MFC) operation. Int J Hydrog Energy 39:10275–10283. CrossRefGoogle Scholar
  16. 16.
    Kondaveeti S, Lee J, Kakarla R, Kim HS, Min B (2014) Low-cost separators for enhanced power production and field application of microbial fuel cells (MFCs). Electrochim Acta 132:434–440. CrossRefGoogle Scholar
  17. 17.
    Venkata Mohan S, Mohanakrishna G, Sarma PN (2010) Composite vegetable waste as renewable resource for bioelectricity generation through non-catalyzed open-air cathode microbial fuel cell. Bioresour Technol 101:970–976. CrossRefPubMedGoogle Scholar
  18. 18.
    Kondaveeti S, Kang E, Liu H, Min B (2019) Continuous autotrophic denitrification process for treating ammonium-rich leachate wastewater in bioelectrochemical denitrification system (BEDS). Bioelectrochemistry 130:107340. CrossRefPubMedGoogle Scholar
  19. 19.
    APHA (2005) Standard methods for the examinatinon of water and wastewater. American Public Health Association, Washington, DCGoogle Scholar
  20. 20.
    Logan BE (2008) Microbial fuel cells. Wiley, HobokemGoogle Scholar
  21. 21.
    Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192. CrossRefPubMedGoogle Scholar
  22. 22.
    Fan Y, Sharbrough E, Liu H (2008) Quantification of the internal resistance distribution of microbial fuel cells. Environ Sci Technol 42:8101–8107. CrossRefPubMedGoogle Scholar
  23. 23.
    Girguis P, Reimers CE (2011) Methane-powered microbial fuel cells. Google PatentsGoogle Scholar
  24. 24.
    He Z, Wagner N, Minteer SD, Angenent LT (2006) An upflow microbial fuel cell with an interior cathode: assessment of the internal resistance by impedance spectroscopy. Environ Sci Technol 40:5212–5217. CrossRefPubMedGoogle Scholar
  25. 25.
    Bond DR, Lovley DR (2003) Electricity production by Geobacter sulfurreducens attached to electrodes. Appl Environ Microbiol 69:1548–1555. CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Bajracharya S, Sharma M, Mohanakrishna G, Dominguez Benneton X, Strik DPBTB, Sarma PM, Pant D (2016) An overview on emerging bioelectrochemical systems (BESs): technology for sustainable electricity, waste remediation, resource recovery, chemical production and beyond. Renew Energy 98:153–170. CrossRefGoogle Scholar
  27. 27.
    Singh HM, Pathak AK, Chopra K, Tyagi VV, Anand S, Kothari R (2018) Microbial fuel cells: a sustainable solution for bioelectricity generation and wastewater treatment. Biofuels 10:11–31. CrossRefGoogle Scholar
  28. 28.
    Wang Z, Mahadevan GD, Wu Y, Zhao F (2017) Progress of air-breathing cathode in microbial fuel cells. J Power Sour 356:245–255. CrossRefGoogle Scholar
  29. 29.
    Santoro C, Arbizzani C, Erable B, Ieropoulos I (2017) Microbial fuel cells: from fundamentals to applications. A review. J Power Sour 356:225–244. CrossRefGoogle Scholar
  30. 30.
    Miran W, Nawaz M, Jang J, Lee DS (2016) Conversion of orange peel waste biomass to bioelectricity using a mediator-less microbial fuel cell. Sci Total Environ 547:197–205. CrossRefPubMedGoogle Scholar
  31. 31.
    Mohanakrishna G, Al-Raoush RI, Abu-Reesh IM (2018) Induced bioelectrochemical metabolism for bioremediation of petroleum refinery wastewater: optimization of applied potential and flow of wastewater. Bioresour Technol 260:227–232. CrossRefPubMedGoogle Scholar
  32. 32.
    Logan BE, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192. CrossRefPubMedGoogle Scholar
  33. 33.
    Oh S, Logan BE (2005) Hydrogen and electricity production from a food processing wastewater using fermentation and microbial fuel cell technologies. Water Res 39:4673–4682. CrossRefPubMedGoogle Scholar
  34. 34.
    Mohanakrishna G, Abu-Reesh IM, Al-Raoush RI, He Z (2018) Cylindrical graphite based microbial fuel cell for the treatment of industrial wastewaters and bioenergy generation. Bioresour Technol 247:753–758. CrossRefPubMedGoogle Scholar
  35. 35.
    Velvizhi G, Babu PS, Mohanakrishna G, Srikanth S, Mohan SV (2012) Evaluation of voltage sag-regain phases to understand the stability of bioelectrochemical system: electro-kinetic analysis. RSC Adv 2(4):1379–1386. CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Division of Chemical EngineeringKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Civil and Architectural Engineering, College of EngineeringQatar UniversityDohaQatar

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