Construction of a Lactobacillus plantarum Strain Expressing the Capsid Protein of Porcine Circovirus Type 2d (PCV2d) as an Oral Vaccine
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Porcine circovirus type 2 (PCV2) is a pathogenic virus that causes high rates of porcine death, resulting in severe economic losses to the swine industry. In recent years, the prevalence of PCV2d genotype infection in pigs has increased, but most commercially available vaccines were developed against the PCV2a strain and do not ensure complete protection from PCV2d. Here, we first constructed an expression vector for the antigenic ORF2-encoded capsid protein of PCV2d (pLp3050-His6-tag-capsid). We then utilized Lactobacillus plantarum to express the protein at mucosal sites in orally vaccinated mice. After transducing L. plantarum with pLp3050-His6-tag-capsid, the expressed protein could be found in cell wall and cell-free supernatant fractions by Western blotting. Using flow cytometry, we found that L. plantarum cells with surface-displayed capsid protein increased with time after SppIP induction. Finally, mice that were orally immunized 18 times with capsid-expressing L. plantarum showed increased levels of capsid-specific sIgA and virus neutralizing activity at mucosal sites, suggesting mucosal immunity had been stimulated by the vaccine. Overall, our findings demonstrate the feasibility and utility of a PCV2d-based vaccine, which may be of great value in porcine agriculture.
KeywordsCapsid protein Lactobacillus plantarum Mucosal immunity Porcine circovirus type 2d sIgA
We thank Dr. Geir Mathiesen (Norwegian University of Life Sciences, Akershus, Norway) for kindly providing pLp3050 plasmid. This study was supported by the fund from the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-002-041).
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest associated with this research.
This animal experiment was approved by the by the Institutional Animal Care and Use Committee of the National Taiwan University (NTU-IACUC/protocol 125/2013). All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the study was conducted.
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