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Indian Journal of Microbiology

, Volume 59, Issue 4, pp 445–450 | Cite as

Insight into the Effect of Small RNA srn225147 on Mutacin IV in Streptococcus mutans

  • Shanshan Liu
  • Huihui Li
  • Zhenfei Guo
  • Junchang Guan
  • Yu SunEmail author
  • Kai ZhangEmail author
Original research article
  • 20 Downloads

Abstract

Streptococcus mutans (S. mutans) is a serious microbe causing dental caries. Mutacin IV is an effective bacteriocin produced by S. mutans to antagonize numerous non-mutans streptococcal species. However, the posttranscriptional regulation of mutacin IV remains unclear. This study aimed to analyze the effect of small RNA srn225147 on mutacin IV. The functional prediction suggested that srn225147 is involved in the production of mutacin IV, an important secondary metabolite. According to RNAhybrid and RNAPredator prediction, the mutacin IV formation-associated gene comD is a target of srn225147. We further analyzed the roles of srn225147 and comD in 20 S. mutans clinical strains with high production of mutacin IV (High-IV group) and lacking mutacin IV (None-IV group). Levels of comD expression were significantly higher in the High-IV group, whereas the Non-IV group showed relatively higher expression of srn225147, with a negative correlation observed between srn225147 and comD. Moreover, compared to the mimic negative control (NC) group, comD expression was decreased at 400-fold srn225147 overexpression but increased at approximately 1400-fold overexpression. Although the production of mutacin IV in the 1400-fold change srn225147 mimic group was larger than that in the 400-fold change mimic group, there was no significant difference in the production of mutacin IV between the srn225147 mimic group and mimic NC group. These results indicate that srn225147 has a two-way regulation effect on the expression of comD but that its regulation in the production of mutacin IV is weak.

Keywords

Streptococcus mutans Mutacin Small RNA comD 

Notes

Acknowledgements

This research was funded by the Natural Science Fund of Education Department of Anhui province, China (Grant Number KJ2018A0223) and the Foundation for Innovative Research Groups of Anhui province, China (2016–40).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed were consistent with the ethical standards of the Ethics Committee of (2017) KY011 from the First Affiliated Hospital of Bengbu Medical College.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.Department of StomatologyThe First Affiliated Hospital of Bengbu Medical CollegeBengbuChina
  2. 2.Department of Microbiology and Anhui Key Laboratory of Infection and ImmunityBengbu Medical CollegeBengbuChina
  3. 3.Department of Biochemistry and Molecular BiologyBengbu Medical CollegeBengbuChina

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