Curcumin Encapsulated PEGylated Nanoliposomes: A Potential Anti-Infective Therapeutic Agent

  • Anuj Mittal
  • Naveen Kumar
  • Nar Singh ChauhanEmail author
Original research article


Exploration of novel bioactive molecules or potentiation of the existing bioactive molecules is necessary to reduce the burden of the infectious diseases for the better human health. Curcumin is a promising molecule with huge therapeutic potential. Despite high bioactivity, its therapeutic suitability is shadowed by poor bioavailability, limited aqueous solubility, and short shelf life. Nanotechnology has generated new avenues to overcome these challenges. In the current study polymer assisted nanoliposomes, PEGylated Curcumin nanoliposomes with good loading efficiency were prepared. These particles have shown 1000 fold enhanced curcumin hydrophilicity and tenfold higher stability. In vitro release kinetic indicates two fold higher curcumin release in the simulated gastric and intestinal environment. Various bioactivity assays have confirmed enhanced bioactivity of nanocurcmin in comparison of the native curcumin. PEGylated Curcumin nanoliposomes could be employed for treating various diseases.


Curcumin Nanoliposomes Antibacterial Antifungal Antioxidant Quorum sensing inhibitors Bioactive 



Funding for this work was obtained from the DBT sponsored research Project BT/PR10801/MED/29/826/2014. Anuj Mittal would like to thank Council of Scientific and Industrial Research (CSIR) for fellowships.

Compliance with Ethical Standards

Conflict of interest

The author declares that they have no conflict of interest.


  1. 1.
    López-Lázaro M (2008) Anticancer and carcinogenic properties of curcumin: considerations for its clinical development as a cancer chemopreventive and chemotherapeutic agent. Mol Nutr Food Res 52:S103–S127. Google Scholar
  2. 2.
    Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB (2007) Bioavailability of curcumin: problems and promises. Mol Pharm 4:807–818. CrossRefGoogle Scholar
  3. 3.
    Wang Y, Lu Z, Wu H, Lv F (2009) Study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. Int J Food Microbiol 136:71–74. CrossRefGoogle Scholar
  4. 4.
    Chauhan NS, Nain S, Sharma R (2017) Identification of arsenic resistance genes from marine sediment metagenome. Indian J Microbiol 57:299–306. CrossRefGoogle Scholar
  5. 5.
    Bisht S, Feldmann G, Soni S, Ravi R, Karikar C, Maitra A, Maitra A (2007) Polymeric nanoparticle-encapsulated curcumin (“nanocurcumin”): a novel strategy for human cancer therapy. J Nanobiotechnol 5:3. CrossRefGoogle Scholar
  6. 6.
    Farnia P, Mollaei S, Bahrami A, Ghassempour A, Velayati AA, Ghanavi J (2016) Improvement of curcumin solubility by polyethylene glycol/chitosan-gelatin nanoparticles (CUR-PEG/CS-G-nps). Biomed Res 27:659–665Google Scholar
  7. 7.
    Ozcelik B, Ho KKK, Glattauer V, Willcox M, Kumar N, Thissen H (2017) Poly(ethylene glycol)-based coatings combining low-biofouling and quorum-sensing inhibiting properties to reduce bacterial colonization. ACS Biomater Sci Eng 3:78–87. CrossRefGoogle Scholar
  8. 8.
    Anand P, Nair HB, Sung B, Kunnumakkara AB, Yadav VR, Tekmal RR, Aggarwal BB (2010) Design of curcumin-loaded PLGA nanoparticles formulation with enhanced cellular uptake, and increased bioactivity in vitro and superior bioavailability in vivo. Biochem Pharmacol 79:330–338. CrossRefGoogle Scholar
  9. 9.
    Kim CY, Bordenave N, Ferruzzi MG, Safavy A, Kim KH (2011) Modification of curcumin with polyethylene glycol enhances the delivery of curcumin in preadipocytes and its antiadipogenic property. J Agric Food Chem 59:1012–1019. CrossRefGoogle Scholar
  10. 10.
    Cheng KK, Yeung CF, Ho SW, Chow SF, Chow AHL, Baum L (2013) Highly stabilized curcumin nanoparticles tested in an in vitro blood–brain barrier model and in Alzheimer’s disease Tg2576 mice. AAPS J 15:324–336. CrossRefGoogle Scholar
  11. 11.
    Li L, Ahmed B, Mehta K, Kurzrock R (2007) Liposomal curcumin with and without oxaliplatin: effects on cell growth, apoptosis, and angiogenesis in colorectal cancer. Mol Cancer Ther 6:1276–1282. CrossRefGoogle Scholar
  12. 12.
    Dadgar N, Esfahani MKM, Torabi S, Alavi SE, Akbarzadeh A (2014) Effects of nanoliposomal and pegylated nanoliposomal artemisinin in treatment of breast cancer. Ind J Clin Biochem 29:501–504. CrossRefGoogle Scholar
  13. 13.
    Hwang H, Jeong H-S, Oh P-S, Kim M, Lee TK, Kwon J, Kim HS, Lim ST, Sohn MH, Jeong HJ et al (2016) PEGylated nanoliposomes encapsulating angiogenic peptides improve perfusion defects: radionuclide imaging-based study. Nucl Med Biol 43:552–558. CrossRefGoogle Scholar
  14. 14.
    Ahmed V, Kumar J, Kumar M, Chauhan MB, Vij M, Ganguli M, Chauhan NS (2013) Synthesis, characterization of penicillin G capped silver nanoconjugates to combat β-lactamase resistance in infectious microorganism. J Biotechnol 163:419–424. CrossRefGoogle Scholar
  15. 15.
    Ahmed V, Kumar J, Kumar M, Chauhan MB, Dahiya P, Chauhan NS (2015) Functionalised iron nanoparticle–penicillin G conjugates: a novel strategy to combat the rapid emergence of β-lactamase resistance among infectious micro-organism. J Exp Nanosci 10:718–728. CrossRefGoogle Scholar
  16. 16.
    Bhawana Basniwal RK, Buttar HS, Jain VK, Jain N (2011) Curcumin nanoparticles: preparation, characterization, and antimicrobial study. J Agric Food Chem 59:2056–2061. CrossRefGoogle Scholar
  17. 17.
    Packiavathy IASV, Agilandeswari P, Musthafa KS, Pandian K, Ravi AV (2012) Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against gram negative bacterial pathogens. Food Res Int 45:85–92. CrossRefGoogle Scholar
  18. 18.
    Ak T, Gülçin İ (2008) Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 174:27–37. CrossRefGoogle Scholar
  19. 19.
    Otari SV, Patel SKS, Kim SY, Haw JR, Kalia VC, Kim IW, Lee JK (2019) Copper ferrite magnetic nanoparticles for the immobilization of enzyme. Indian J Microbiol 59:105–108. CrossRefGoogle Scholar
  20. 20.
    Patel SKS, Jeon MS, Gupta RK, Jeon Y, Kalia VC, Kim SC, Cho BK, Kim DR, Lee JK (2019) Hierarchical macroporous particles for efficient whole-cell immobilization: application in bioconversion of greenhouse gases to methanol. ACS Appl Mater Interfaces. Google Scholar
  21. 21.
    Ahmed V, Kumar M, Kumar J, Chauhan MB, Chauhan NS (2014) Nanogold/polyaniline/penicillin G nanoconjugates: a novel nanomedicine. Int J Polym Mater Polym Biomater 63:86–91. CrossRefGoogle Scholar
  22. 22.
    Tao N (2019) Challenges and promises of metal oxide nanosensors. ACS Sens 4:780. CrossRefGoogle Scholar
  23. 23.
    Chen X, Zou L-Q, Niu J, Liu W, Peng SF, Liu CM (2015) the stability, sustained release and cellular antioxidant activity of curcumin nanoliposomes. Molecules 20:14293–14311. CrossRefGoogle Scholar
  24. 24.
    Yatuv R, Robinson M, Dayan-Tarshish I, Baru M (2010) The use of PEGylated liposomes in the development of drug delivery applications for the treatment of hemophilia. Int J Nanomedicine 5:581–591. Google Scholar
  25. 25.
    Shin GH, Chung SK, Kim JT, Joung HJ, Park HJ (2013) Preparation of chitosan-coated nanoliposomes for improving the mucoadhesive property of curcumin using the ethanol injection method. J Agric Food Chem 61:11119–11126. CrossRefGoogle Scholar
  26. 26.
    Ha PT, Le MH, Hoang TMN, Le TTH, Duong TQ, Tran THH, Tran DL, Nguyen XP (2012) Preparation and anti-cancer activity of polymer-encapsulated curcumin nanoparticles. Adv Nat Sci: Nanosci Nanotechnol 3:035002. Google Scholar
  27. 27.
    Dhule SS, Penfornis P, He J, Harris MR, Terry T, John V, Pochampally R (2014) The combined effect of encapsulating curcumin and C6 ceramide in liposomal nanoparticles against osteosarcoma. Mol Pharm 11:417–427. CrossRefGoogle Scholar
  28. 28.
    Nguyen TTH, Si J, Kang C, Chung B, Chung D, Kim D (2017) Facile preparation of water soluble curcuminoids extracted from turmeric (Curcuma longa L.) powder by using steviol glucosides. Food Chem 214:366–373. CrossRefGoogle Scholar
  29. 29.
    Geng S, Yang B, Wang G, Qin G, Wada S, Wang JY (2014) Two cholesterol derivative-based PEGylated liposomes as drug delivery system, study on pharmacokinetics and drug delivery to retina. Nanotechnology 25:275103. CrossRefGoogle Scholar
  30. 30.
    Yen FL, Wu T-H, Tzeng CW, Lin LT, Lin CC (2010) Curcumin nanoparticles improve the physicochemical properties of curcumin and effectively enhance its antioxidant and antihepatoma activities. J Agric Food Chem 58:7376–7382. CrossRefGoogle Scholar
  31. 31.
    Cabral H, Matsumoto Y, Mizuno K, Chen Q, Murakami M, Kimura M, Terada Y, Kano MR, Miyazono K, Uesaka M, Nishiyama N, Kataoka K (2011) Accumulation of sub-100 nm polymeric micelles in poorly permeable tumours depends on size. Nat Nanotech 6:815–823. CrossRefGoogle Scholar
  32. 32.
    Kalia VC (2013) Quorum sensing inhibitors: an overview. Biotechnol Adv 31:224–245. CrossRefGoogle Scholar
  33. 33.
    Kalia VC (2014) Microbes, antimicrobials and resistance: the battle goes on. Indian J Microbiol 54:1–2. CrossRefGoogle Scholar
  34. 34.
    Kalia VC (2017) The dawn of the era of bioactive compounds. In: Kalia VC, Saini AK (eds) Metabolic engineering for bioactive compounds. Springer, Singapore, pp 3–10. CrossRefGoogle Scholar
  35. 35.
    Kalia VC, Patel SKS, Kang YC, Lee JK (2019) Quorum sensing inhibitors as antipathogens: biotechnological applications. Biotechnol Adv 37:68–90. CrossRefGoogle Scholar
  36. 36.
    Kalia VC, Prakash J, Koul S, Ray S (2017) Simple and rapid method for detecting biofilm forming bacteria. Indian J Microbiol 57:109–111. CrossRefGoogle Scholar
  37. 37.
    Kalia VC, Purohit HJ (2011) Quenching the quorum sensing system: potential antibacterial drug targets. Crit Rev Microbiol 37:121–140. CrossRefGoogle Scholar
  38. 38.
    Koul S, Kalia VC (2017) Multiplicity of quorum quenching enzymes: a potential mechanism to limit quorum sensing bacterial population. Indian J Microbiol 57:100–108. CrossRefGoogle Scholar
  39. 39.
    Chavanpatil MD, Khdair A, Patil Y, Handa H, Mao G, Panyam J (2007) Polymer-surfactant nanoparticles for sustained release of water soluble drugs. J Pharm Sci 96:3379–3389. CrossRefGoogle Scholar
  40. 40.
    Neelofar K, Shreaz S, Rimple B, Muralidhar S, Nikhat M, Khan LA (2011) Curcumin as a promising anticandidal of clinical interest. Can J Microbiol 57:204–210. CrossRefGoogle Scholar
  41. 41.
    Jovanovic SV, Steenken S, Boone CW, Simic MG (1999) H-atom transfer is a preferred antioxidant mechanism of curcumin. J Am Chem Soc 121:9677–9681. CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  • Anuj Mittal
    • 1
    • 2
  • Naveen Kumar
    • 2
  • Nar Singh Chauhan
    • 1
    Email author
  1. 1.Department of BiochemistryMaharshi Dayanand UniversityRohtakIndia
  2. 2.Department of ChemistryMaharshi Dayanand UniversityRohtakIndia

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