Indian Journal of Microbiology

, Volume 59, Issue 2, pp 234–236 | Cite as

Importance of the 3′-Terminal Nucleotide of the Forward Primer for Nucleoprotein Gene Detection of Viral Hemorrhagic Septicemia Virus by Conventional Reverse-Transcription PCR

  • Hyoung Jun KimEmail author
Short Communications


Viral hemorrhagic septicemia virus (VHSV), causing severe diseases in farmed fish, is detected and genotyped using conventional reverse-transcription PCR (cRT-PCR) targeting the nucleoprotein gene with corresponding VN F (forward) and VN R (reverse) primers. However, these primers have low sensitivity to VHSV subtype IVa; I investigated the cause for the poor cRT-PCR performance using various primer combinations. The results demonstrated that a 3′-end mismatch in the VN F primer reduced sensitivity and plays a critical role in VHSV detection by cRT-PCR.


Viral hemorrhagic septicemia virus Low sensitivity Conventional RT-PCR 3′-Terminal region of forward primer 



I thank Niels Jørgen Olesen and Argelia Cuenca for technical and scientific support.


This study was funded by the National Fishery Products Quality Management Service, Republic of Korea.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Association of Microbiologists of India 2019

Authors and Affiliations

  1. 1.OIE Reference Laboratory for Viral Hemorrhagic Septicemia (VHS)National Fishery Products Quality Management ServicesBusanKorea

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