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Indian Journal of Microbiology

, Volume 59, Issue 2, pp 241–245 | Cite as

Changes in Gene Expression in Human Epithelial and Mast Cells in Response to Vesicles from Klebsiella pneumonia ATCC 13883

  • Hee Sang You
  • Yeon Jeong Ok
  • Song Hee Lee
  • Sang Sun Kang
  • Sung Hee HyunEmail author
Short Communications
  • 124 Downloads

Abstract

We investigated alterations in the expression of immune-related genes in epithelial cells and mast cells treated with outer membrane vesicles (OMVs) derived from Klebsiella pneumoniae (KpOMVs). Previous studies have shown that OMVs contain substances that enable their delivery to host cells and induce an immune response. Our results indicate an increase in expression of genes such as IL-8, IL-1b, MIP-, HMOX1, HSPA1A, and IL-24 in epithelial cells and mast cells treated with KpOMVs. The pathogenicity of KpOMVs was confirmed by measuring the changes in the expression of these immune-related genes.

Keywords

Klebsiella pneumoniae Immune related genes Outer membrane vesicles Pathogen Toxin 

Notes

Acknowledgements

This research was supported by the Basic Science Research Program, through the National Research Foundation of Korea (NRF), funded by the Ministry of Science ICT & Future Planning (NRF-2014R1A1A1002349).

Compliance with Ethical Standards

Conflict of interest

The authors have no conflict of interest (commercial or otherwise) to declare regarding this study.

References

  1. 1.
    Ng WL, Bassler BL (2009) Bacterial quorum-sensing network architectures. Annu Rev Genet 43:197–222CrossRefGoogle Scholar
  2. 2.
    Mashburn-Warren LM, Whiteley M (2006) Special delivery: vesicle trafficking in prokaryotes. Mol Microbiol 61:839–846CrossRefGoogle Scholar
  3. 3.
    Kuehn MJ, Kesty NC (2005) Bacterial outer membrane vesicles and the host-pathogen interaction. Genes Dev 19:2645–2655CrossRefGoogle Scholar
  4. 4.
    Kulp A, Kuehn MJ (2010) Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu Rev Microbiol 64:163–184CrossRefGoogle Scholar
  5. 5.
    MacDonald KL, Beveridge TJ (2002) Bactericidal effect of gentamicin-induced membrane vesicles derived from Pseudomonas aeruginosa PAO1 on Gram-positive bacteria. Can J Microbiol 48:810–820CrossRefGoogle Scholar
  6. 6.
    Lindmark B, Rompikuntal PK, Vaitkevicius K, Song T, Mizunoe Y, Uhlin BE, Guerry P, Wai SN (2009) Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuni. BMC Microbiol 9:220CrossRefGoogle Scholar
  7. 7.
    Wai SN, Lindmark B, Soderblom T, Takade A, Westermark M, Oscarsson J, Jass J, Richter-Dahlfors A, Mizunoe Y, Uhlin B (2003) Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin. Cell 115:25–35CrossRefGoogle Scholar
  8. 8.
    Bouza E, Cercenado E (2002) Klebsiella and enterobacter: antibiotic resistance and treatment implications. Semin Respir Infect 17:215–230CrossRefGoogle Scholar
  9. 9.
    Keynan Y, Rubinstein E (2007) The changing face of Klebsiella pneumoniae infections in the community. Int J Antimicrob Agents 30:385–389CrossRefGoogle Scholar
  10. 10.
    Cortés G, Borrell N, de Astorza B, Gómez C, Sauleda J, Albertí S (2002) Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia. Infect Immun 70:2583–2590CrossRefGoogle Scholar
  11. 11.
    Shankar-Sinha S, Valencia GA, Janes BK, Rosenberg JK, Whitfield C, Bender RA, Standiford TJ, Younger JG (2004) The Klebsiella pneumoniae O antigen contributes to bacteremia and lethality during murine pneumonia. Infect Immun 72:1423–1430CrossRefGoogle Scholar
  12. 12.
    Lawlor MS, Hsu J, Rick PD, Miller VL (2005) Identification of Klebsiella pneumoniae virulence determinants using an intranasal infection model. Mol Microbiol 58:1054–1073CrossRefGoogle Scholar
  13. 13.
    Cano V, Moranta D, Llobet-Brossa E, Bengoechea JA, Garmendia J (2009) Klebsiella pneumoniae triggers a cytotoxic effect on airway epithelial cells. BMC Microbiol 9:156CrossRefGoogle Scholar
  14. 14.
    Straus DC (1987) Production of an extracellular toxic complex by various strains of Klebsiella pneumonia. Infect Immun 55:44–48Google Scholar
  15. 15.
    Henry T, Pommier S, Journet L, Bernadac A, Grovel JP, Lloubes R (2004) Improved methods for producing outer membrane vesicles in gram-negative bacteria. Res Microbiol 155:437–446CrossRefGoogle Scholar
  16. 16.
    You HS, Kim MJ, OK YJ, Lee EJ, Kang SS, Hyun SH (2018) Characteristics of outer-membrane vesicles derived from Klebsiella pneumonia ATCC 13883. IJCMPR 4:3171–3176Google Scholar
  17. 17.
    Lee JC, Lee EJ, Lee JH, Jun SH, Choi CW, Kim SI, Kang SS, Hyun SH (2012) Klebsiella pneumoniae secretes outer membrane vesicles that induce the innate immune response. FEMS Microbiol Lett 331:17–24CrossRefGoogle Scholar
  18. 18.
    Uli L, Castellanos-Serra L, Betancourt L, Dominguez F, Barbera R, Sotolongo F, Guillen G, Pajon Feyt R (2006) Outer membrane vesicles of the VA-MENGOC-BC vaccine against serogroup B of Neisseria meningitides: analysis of protein components by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 6:3389–3399CrossRefGoogle Scholar
  19. 19.
    Renelli M, Matias V, Lo RY, Beveridge TJ (2004) DNA-containing membrane vesicles of Pseudomonas aeruginosa PAO1 and their genetic transformation potential. Microbiology 150:2161–2169CrossRefGoogle Scholar
  20. 20.
    Bauman SJ, Kuehn MJ (2006) Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response. Microbes Infect 8:2400–2408CrossRefGoogle Scholar
  21. 21.
    Piantadosi CA, Withers CM, Bartz RR, MacGarvey NC, Fu P, Sweeney TE, Welty-Wolf KE, Suliman HB (2011) Heme oxygenase-1 couples activation of mitochondrial biogenesis to anti-inflammatory cytokine expression. J Biol Chem 286:16374–16385CrossRefGoogle Scholar
  22. 22.
    Wang M, Liang P (2005) Interleukin-24 and its receptors. Immunology 114:166–170CrossRefGoogle Scholar

Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.Department of Biomedical Laboratory Science, School of MedicineEulji UniversityDaejeonRepublic of Korea
  2. 2.BK21 Plus Program, Department of Senior Healthcare, Graduate SchoolEulji UniversityDaejeonRepublic of Korea
  3. 3.School of Science EducationChungbuk National UniversityChongjuRepublic of Korea

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