Detection of Wolbachia Phage (WO) in Indian Lac Insect [Kerria lacca (Kerr.)] and Its Implications
Wolbachia, a maternally inherited bacterium induces reproductive alterations in its hosts such as feminization of males, male killing and parthenogenesis. It is the most diverse endosymbiont infecting more than 70% of the insects ranging from pests to pollinators. Kerria lacca—a hemipteran is a sedentary, oriental insect known to produce lac—the only resin of animal origin. The present study was conducted to screen the presence of Wolbachia and its associated phages in the two infrasubspecific forms (four insect lines) of K. lacca viz. kusmi and rengeeni differing from each other on the basis of host preference. Wolbachia and its associated phage were found to be prevalent in all the insect lines analyzed. We, hereby, report the presence of WO-phage (Wolbachia phage) for the first time in K. lacca. Further, phylogenetic data differentiated the kusmi and rengeeni infrasubspecific forms into two different groups on the basis of WO-phage sequences.
KeywordsWolbachia phage Wolbachia Kerria lacca Lac
Financially support by NAIP-ICAR, New Delhi and R&D Grant by University of Delhi is gratefully acknowledged.
- 1.Werren JH, O’Neill SL (1997) The evolution of heritable symbionts. In: O’Neill SL, Hoffmann AA, Werren JH (eds) Influential passengers: inherited microorganisms and arthropod reproduction. Oxford University Press, Oxford, pp 1–41Google Scholar
- 6.Hurst GD, Schulenburg JHG, Majerus TM, Bertrand D, Zakharov IA, Baungaard J, Volkl W, Stouthamer R, Majerus ME (1999) Invasion of one insect species, Adalia bipunctata by two different male-killing bacteria. Insect Mol Biol 8:133–139. https://doi.org/10.3201/eid0604.000402 CrossRefPubMedGoogle Scholar
- 11.Wu M, Sun LV, Vamathevan J, Riegler M, Deboy R, Brownlie JC, McGraw EA, Martin W, Esser C, Ah-madinejad N (2004) Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. PLoS Biol 2:0327–0341. https://doi.org/10.1371/journal.pbio.0020069 CrossRefGoogle Scholar
- 13.Ros VI, Fleming VM, Feil EJ, Breeuwer JA (2009) How diverse is the genus Wolbachia? Multiple-gene sequencing reveals a putatively new Wolbachia supergroup recovered from spider mites (Acari: Tetranychidae). Appl Environ Microbiol 75:1036–1043. https://doi.org/10.1128/AEM.01109-08 CrossRefPubMedGoogle Scholar
- 17.Sharma KK, Ramani R (1999) An update on synoptic catalogue of lac insects (Homoptera: Tachardiidae). J Bombay Nat Hist Soc 96:438–443Google Scholar
- 18.Sharma KK, Jaiswal AK, Kumar KK (2006) Role of lac culture in biodiversity conservation: issue at stake and conservation strategy. Curr Sci 91:894–898Google Scholar