Indian Journal of Microbiology

, Volume 59, Issue 1, pp 116–120 | Cite as

In Vitro Antibacterial and Synergistic Effect of Chitosan-Phytochemical Conjugates Against Antibiotic Resistant Fish Pathogenic Bacteria

  • Grace N. A. Charway
  • Seulki Park
  • Daeung Yu
  • Jae-Young Je
  • Do-Hung Kim
  • Won-Kyo Jung
  • Young-Mog KimEmail author
Short Communication


Chitosan-phytochemical conjugates exhibited significant antibacterial effect with minimum inhibitory concentration (MIC) ranging from 128 to 2048 µg/ml against antibiotic-resistant fish pathogenic bacteria such as Edwardseilla tarda, Vibrio harveyi and Photobacterium damselaewhich were isolated from Korean cultured fish. Furthermore, the MIC values of old-fashioned antibiotics such as erythromycin and oxytertacycline drastically reduced in combination with chitosan-phytochemical conjugates against the fish pathogenic bacteria. The combination of conjugates with erythromycin and oxytetracycline gave median ∑FIC results ranging from 0.281 to 0.625 and 0.312 to 0.625, respectively. This result indicates the synergistic antibacterial effects and an increased susceptibility against the antibiotics.


Antibacterial effect Chitosan-phytochemical conjugate Fish pathogenic bacteria Synergy effect 



This research was funded by the Marine Biotechnology Program (20150220) funded by the Ministry of Oceans and Fisheries, Republic of Korea. The pathogen for this study was provided by the Gyeongsang National University Hospital Branch of National Culture Collection for pathogens (GNUH-NCCP).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Xu T, Zhang XH (2014) Edwardsiella tarda: an intriguing problem in aquaculture. Aquaculture 431:129–135. CrossRefGoogle Scholar
  2. 2.
    Hervio-Heath D, Colwell RR, Derrien A, Robert-Pillot A, Fournier JM, Pommepuy M (2002) Occurrence of pathogenic vibrios in coastal areas of France. J Appl Microbiol 92:1123–1135. CrossRefGoogle Scholar
  3. 3.
    Sawabe T, Fujimura Y, Niwa K, Aono H (2007) Vibrio comitans sp. nov., Vibrio rarus sp. nov. and Vibrio inusitatus sp. nov., from the gut of the abalones Haliotis discus discus, H. gigantea, H. madaka and H. rufescens. Int J Syst Evol Microbiol 57:916–922. CrossRefGoogle Scholar
  4. 4.
    Koul S, Prakash J, Mishra A, Kalia VC (2016) Potential emergence of multi-quorum sensing inhibitor resistant (MQSIR) bacteria. Indian J Microbiol 56:1–18. CrossRefGoogle Scholar
  5. 5.
    Kim JH, Yu D, Eom SH, Kim SH, Oh J, Jung WK, Kim YM (2017) Synergistic antibacterial effects of chitosan-caffeic acid conjugate against antibiotic-resistant acne-related bacteria. Mar Drugs 15:167. CrossRefGoogle Scholar
  6. 6.
    Kim KH, Yu D, Eom SH, Kim HJ, Kim DH, Song HS, Kim DM, Kim YM (2018) Fucofuroeckol: a from edible marine alga Eisenia bicyclis to restore antifungal effect of fluconazole against fluconazole-resistant Candida albicans. J Appl Phycol 30:605–609. CrossRefGoogle Scholar
  7. 7.
    Cho YS, Kim SK, Ahn CB, Je JY (2011) Preparation, characterization, and antioxidant properties of gallic acid-grafted-chitosans. Carbohydr Polym 83:1617–1622. CrossRefGoogle Scholar
  8. 8.
    Eom SH, Kang SK, Lee DS, Myeong JI, Lee JH, Kim HW, Kim KH, Je JY, Jung WK, Kim YM (2016) Synergistic antibacterial effect and antibacterial action mode of chitosan-ferulic acid conjugate against methicillin-resistant Staphylococcus aureus. J Microbiol Biotechnol 26:784–789. CrossRefGoogle Scholar
  9. 9.
    Aytekin AZ, Morimura S, Kida K (2011) Synthesis of chitosan-caffeic acid derivatives and evaluation of their antioxidant activities. J Biosci Bioeng 111:212–216. CrossRefGoogle Scholar
  10. 10.
    Lee DS, Woo JY, Ahn CB, Je JY (2014) Chitosan–hydroxycinnamic acid conjugates: preparation, antioxidant and antimicrobial activity. Food Chem 148:97–104. CrossRefGoogle Scholar
  11. 11.
    Koul S, Kalia VC (2017) Multiplicity of quorum quenching enzymes: a potential mechanism to limit quorum sensing bacterial population. Indian J Microbiol 57:100–108. CrossRefGoogle Scholar
  12. 12.
    Kwon YI, Apostolidis E, Labbe RG, Shetty K (2007) Inhibition of Staphylococcus aureus by phenolic phytochemicals of selected clonal herbs species of Lamiaceae family and likely mode of action through proline oxidation. Food Biotechnol 21:71–89. CrossRefGoogle Scholar
  13. 13.
    Lim J, Hwang Y, Park B, Yun H (2003) Combination effects of cephalexin and gentamicin on Edwardsiella tarda and Streptococcus iniae. Int J Antimicrob Agents 22:67–69. CrossRefGoogle Scholar
  14. 14.
    Xiao BZ, Chen X, Xiang CB, Tang N, Zhang QF, Xiong LZ (2009) Evaluation of seven function-known candidate genes for their effects on improving drought resistance of transgenic rice under field conditions. Mol Plant 2:73–83. CrossRefGoogle Scholar
  15. 15.
    Stock I, Wiedemann B (2001) Natural antibiotic susceptibilities of Edwardsiella tarda, E. ictaluri, and E. hoshinae. Antimicrob Agents Chemother 45:2245–2255. CrossRefGoogle Scholar
  16. 16.
    Scarano C, Spanu C, Ziino G, Pedonese F, Dalmasso A, Spanu V, Virdis S, De Santis EP (2014) Antibiotic resistance of Vibrio species isolated from Sparus aurata reared in Italian mariculture. New Microbiol 37:329–337Google Scholar
  17. 17.
    Choi MS, Park KH, Choi SH (1995) Drug resistance and R plasmids in Edwardsiella tarda from cultured eel. Int J Aquac Fish Sci Res 11:141–150Google Scholar
  18. 18.
    Miller RA, Harbottle H (2017) Antimicrobial drug resistance in fish pathogens. Microbiol Spectr. Google Scholar

Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  1. 1.KOICA-PKNU International Graduate Program of Fisheries SciencePukyong National UniversityBusanKorea
  2. 2.Department of Food Science and TechnologyPukyong National UniversityBusanKorea
  3. 3.Institute of Food Industrialization, Institutes of Green Bio Science and TechnologySeoul National UniversityPyeongchangKorea
  4. 4.Department of Marine-Bio Convergence SciencePukyong National UniversityBusanKorea
  5. 5.Department of Aquatic Life MedicinePukyong National UniversityBusanKorea
  6. 6.Department of Biomedical EngineeringPukyong National UniversityBusanKorea
  7. 7.Marine-Integrated Bionics Research CenterPukyong National UniversityBusanKorea

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