Indian Journal of Microbiology

, Volume 58, Issue 2, pp 138–145 | Cite as

Virulence Factors in Candida albicans and Streptococcus mutans Biofilms Mediated by Farnesol

  • Renan Aparecido Fernandes
  • Douglas Roberto Monteiro
  • Laís Salomão Arias
  • Gabriela Lopes Fernandes
  • Alberto Carlos Botazzo Delbem
  • Debora Barros Barbosa
Original Research Article

Abstract

The aim of this study was to evaluate the effect of farnesol on the production of acids and hydrolytic enzymes by biofilms of Streptococcus mutans and Candida albicans. The present study also evaluated the time-kill curve and the effect of farnesol on matrix composition and structure of single-species and dual-species biofilms. Farnesol, at subinhibitory concentrations, showed a significant reduction in S. mutans biofilm acid production, but did not alter C. albicans hydrolytic enzyme production. The number of cultivable cells of both microorganisms was significantly reduced after 8 h of contact with farnesol. Extracellular matrix protein content was reduced for biofilms formed in the presence of farnesol. In addition, confocal laser scanning and scanning electron microscopy displayed structural alterations in all biofilms treated with farnesol, which included reduction in viable cells and extracellular matrix. In conclusion, farnesol showed favorable properties controlling some virulence factors of S. mutans and C. albicans biofilms. These findings should stimulate further studies using this quorum-sensing molecule, combined with other drugs, to prevent or treat biofilm-associated oral diseases.

Keywords

Biofilms Fungi Quorum sensing Streptococci Virulence 

Notes

Acknowledgements

The authors thank George Duchow for the English review of the manuscript. This study was supported by the São Paulo Research Foundation (FAPESP, Grant Number 2013/23592-0), Brazil.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Association of Microbiologists of India 2018

Authors and Affiliations

  • Renan Aparecido Fernandes
    • 1
  • Douglas Roberto Monteiro
    • 2
  • Laís Salomão Arias
    • 3
  • Gabriela Lopes Fernandes
    • 4
  • Alberto Carlos Botazzo Delbem
    • 3
  • Debora Barros Barbosa
    • 4
  1. 1.Department of DentistryUniversity Center of Adamantina (UNIFAI)Adamantina, São PauloBrazil
  2. 2.Graduate Program in Dentistry (GPD - Master’s Degree)University of Western São Paulo (UNOESTE)Presidente Prudente, São PauloBrazil
  3. 3.Department of Pediatric Dentistry and Public HealthSão Paulo State University (UNESP), School of DentistryAraçatuba, São PauloBrazil
  4. 4.Department of Dental Materials and ProsthodonticsSão Paulo State University (UNESP), School of DentistryAraçatuba, São PauloBrazil

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