Co-digestion of Biowastes to Enhance Biological Hydrogen Process by Defined Mixed Bacterial Cultures
- 116 Downloads
Co-digestion of biowastes for hydrogen (H2) production using defined mixed cultures can overcome the high risk of failure due to contamination and imbalanced nutrient status. H2 production from biowastes—pea-shells, potato peels (PP), onion peels (OP) and apple pomace, either individually or in various combinations was evaluated by hydrolyzing with defined hydrolytic mixed bacterial culture (MHC5) and subjecting the hydrolysate to mixture of defined H2 producers (MMC6). Co-digestion of OP and PP hydrolysate supplemented at H2 production stage with GM-2 and M-9 media resulted in 95 and 102 l H2/kg of Total solids (TS), respectively compared to 84 l H2/kg of TS in control. Upscaling the process by digesting 4.0 l slurry (16-fold) resulted in 88.5 and 95 l H2/kg of TS, respectively compared to 72 l H2/kg of TS in control. Thus, H2 production by co-digestion of biowastes could be improved through the supplementation with very dilute medium (0.1 ×) and selection of suitable biowastes under unsterile conditions. The overall efficiency can be further enhanced by integrating it with bioprocesses for biopolymers such as polyhydroxyalkanoates and or biofuels like methane production.
KeywordsBiowaste Bacillus Hydrogen Onion peels Potato peels Mixed microbial culture
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2018H1D3A2001746, 2013M3A6A8073184). This research was also supported by KU Research Professor Program of Konkuk University.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
- 4.Sitthikitpanya S, Reungsang A, Prasertsan P (2018) Two-stage thermophilic bio-hydrogen and methane production from lime-pretreated oil palm trunk by simultaneous saccharification and fermentation. Int J Hydrog Energy 43:4284–4293. https://doi.org/10.1016/j.ijhydene.2018.01.063 CrossRefGoogle Scholar
- 6.Ding L, Gutierrez EC, Cheng J, Xia A, O’Shea R, Guneratnam AJ, Murphy JD (2018) Assessment of continuous fermentative hydrogen and methane co-production using macro- and micro-algae with increasing organic loading rate. Energy 151:760–770. https://doi.org/10.1016/j.energy.2018.03.103 CrossRefGoogle Scholar
- 16.Porwal S, Kumar T, Lal S, Rani A, Kumar S, Cheema S, Purohit HJ, Sharma R, Patel SKS, Kalia VC (2008) Hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process. Bioresour Technol 99:5444–5451. https://doi.org/10.1016/j.biortech.2007.11.011 CrossRefGoogle Scholar
- 27.Singh G, Arya SK, Gupta V, Sharma P (2017) Enzyme technology for lignocellulosic biomass conversion and recycling to valuable paper and other products: challenges ahead. J Mol Biol Technol 2:105Google Scholar