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Indian Journal of Microbiology

, Volume 51, Issue 1, pp 8–13 | Cite as

RETRACTED ARTICLE: Ecological Significance of Microdiversity: Coexistence Among Casing Soil Bacterial Strains Through Allocation of Nutritional Resource

  • Devendra Kumar ChoudharyEmail author
  • Bhavdish N. Johri
Original Article

Abstract

A combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16S rRNA gene sequences can represent distinct eco- and genotypes. A set of eight bacterial strains wherein three were Pseudomonas putida and rest were Acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. These strains had identical 16S rRNA gene sequences and represented the dominant phylotype in the plateable fraction. Each strain utilized a specific combination of 154 carbon substrates, and the niche overlap indices were low, suggesting that each strain occupied a different ecological niche. Our results have implications for assessment of the diversity and biogeography of bacteria and increase the perception of natural diversity beyond the level of 16S rRNA gene sequences. It is worthwhile approach to explore prokaryotic diversity in different ecological niches.

Keywords

Microdiversity Ecophysiology 16S rRNA sequencing Pseudomonas putida Acinetobacter calcoaceticus 

Notes

Acknowledgments

This work was performed at Department of Biotechnology, BU, Bhopal with kind cooperation of Dr. Anil Prakash, Head, Department of Biotechnology & Bioinformatics Centre, Bhopal.

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Copyright information

© Association of Microbiologists of India 2011

Authors and Affiliations

  1. 1.Department of Science, Faculty of Arts, Science & Commerce (FASC), Mody Institute of Technology & Science (MITS)SikarIndia
  2. 2.Department of Biotechnology & Bioinformatics CentreBarkatullah UniversityBhopalIndia

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