Laser capture microdissection: techniques and applications in liver diseases
Routine transcriptomic and proteomic analysis are usually performed at a whole organ or tissue level. These approaches provide an average readout of all cell types present within the tissue but do not allow differentiating the profile of specific cell populations. Laser capture microdissection (LCM) constitutes an excellent tool to isolate cell populations or areas of interest within a tissue. By direct visualization, the selected area is excised by a laser and can be further processed for a variety of downstream analyses. This technology has been widely used in the study of liver diseases, from DNA and RNA sequencing to mass spectrometry. However, LCM also has important limitations. To ensure the best integrity of the molecule of interest, optimal tissue preservation, careful tissue sectioning, and optimization of the staining procedure are required. The present review provides a description of the LCM technology, including tips and technical recommendations to perform the procedure, as well as an overview of studies using LCM technology in the field of liver disease.
KeywordsLaser capture microdissection Liver diseases Tissue preservation Transcriptome Proteome Downstream analysis
The work was funded by grants from Instituto de Salud Carlos III (PI17/00673), Miguel Servet (CP/00041) and PFIS (FI16/00203), co-financed by Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea, ‘Una manera de hacer Europa’ and The European Foundation for Alcohol Research (ERAB) Grant EA1653.
Compliance with ethical standards
The work was funded by grants from the Instituto de Salud Carlos III (PI17/00673), Miguel Servet (CP/00041) and PFIS (FI16/00203), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER), Unión Europea, ‘Una manera de hacer Europa’ and The European Foundation for Alcohol Research (ERAB) Grant EA1653.
Conflict of interest
The authors declare no conflict of interest.
This article does not contain any study with human participants or animals performed by any of the authors.
- 1.Kandathil AJ, Graw F, Quinn J, Hwang HS, Torbenson M, Perelson AS, et al. Use of laser capture microdissection to map hepatitis C virus-positive hepatocytes in human liver. Gastroenterology 2013;145(1404–1413):e10Google Scholar
- 13.Gallagher RI, Blakely SR, Liotta LA, Espina V. Laser capture microdissection: ArcturusXT infrared capture and UV cutting methods. New York: Humana Press; 2012. pp. 157–178Google Scholar
- 22.Chiu K-W, Nakano T, Chen K-D, Hu T-H, Lin C-C, Hsu L-W, et al. Identification of IL-28B genotype modification in hepatocytes after living donor liver transplantation by laser capture microdissection and pyrosequencing analysis. Biomed Res Int 2018;2018:1–8Google Scholar
- 44.Andersen JB, Spee B, Blechacz BR, Avital I, Komuta M, Barbour A, et al. Genomic and genetic characterization of cholangiocarcinoma identifies therapeutic targets for tyrosine kinase inhibitors. Gastroenterology 2012;142(1021–1031):e15 (NIH Public Access) Google Scholar