Evaluation of Stromal Myofibroblasts in Laryngeal Dysplasia and Invasive Squamous Cell Carcinoma
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Malignant tumors consist of both carcinoma cells and tumor associated host cells. Host cells has started to receive more attention regarding their role in tumor progression such as invasion and metastasis. Fibroblasts that are incorporated in the tumoral stroma are called as peritumoral fibroblasts, reactive stroma, cancer-related fibroblasts or myofibroblasts. In general fibroblasts next to nich of cancer cells express alpha–smooth muscle actin (α-SMA) which is an important marker for differentiated myofibroblasts. Our aim is to investigate the role of α-SMA positive myofibroblasts in the development and progression of laryngeal carcinoma. The proportion of α-SMA positive myofibroblasts are scored from (1 +) to 3( +) in laryngeal dysplasia (n = 17) and microinvasive and invasive squamous cell carcinoma (n = 66). α-SMA expression in invasive carcinoma and dysplasia was analyzed statistically. No stromal myofibroblast was detected in mild dysplasia (score 0). Among 12 cases of moderate-severe dysplasia, in only 3 cases low α-SMA expression (score 1) was observed and in 9 cases there were no stromal myofibroblasts (score 0) were. In most cases of invasive carcinoma, high α-SMA expression (score 2, 3) was seen. α-SMA positive stromal myofibroblasts were significantly higher in invasive laryngeal squamous cell carcinoma compared to dysplasia (p < 0.05). Results of our study suggested that α-SMA positive stromal myofibroblasts play an important role in creating the permissive environment for tumor invasion in laryngeal squamous cell carcinoma. According to this data, we think that the presence of stromal myofibroblasts might be used as a helpful criterion in the differential diagnosis of true invasion and pseudoinvasion.
KeywordsMyofibroblast Larynx Squamous cell carcinoma Laryngeal dysplasia Actin
AOC: planning the study, revaluation of histopathologic diagnosis of all the cases; evaluation of the results, writing the manuscript and financial support of the study. YD: review and editing of the manuscript, evaluation of the results. AS: planning the study, evaluation of the results and financial support of the study. FD: evaluation of the results. IO: evaluation of the results. KI: providing the clinical data and providing the specimens for control group.
- 1.Kirchner JA, Carter D (2004) Pathology of the larynx. In: Mills SE (ed) Sternberg’s diagnostic surgical pathology, 4th edn. Gopson Papers Ltd, Noida, pp 1007–1032Google Scholar
- 2.Lingen MW (2015) Head and neck. In: Kumar V, Abbas AK, Aster JC (eds) Robbins and cotran pathologic basis of disease, 9th edn. Elsevier Saunders, Philadelphia, pp 738–740Google Scholar
- 8.Powell DW, Adegboyega PA, DiMari JF, Mifflin RC (2005) Epithelial cells and their neighbors. I. Role of intestinal myofibroblasts in development, repair, and cancer. Am J Physiol-Gastr L 289:G2–G7Google Scholar
- 17.Chaudhary M, Gadbail AR, Vidhale G, Mankar MP, Gondivkar SM, Gawande M et al (2012) Comparison of myofibroblasts expression in oral squamous cell carcinoma, verrucous carcinoma, high risk epithelial dysplasia, low risk epithelial dysplasia and normal oral mucosa. Head Neck Pathol 6:305–313CrossRefGoogle Scholar
- 27.Seifi S, Shafaei S, Shafigh E, Sahabi SM, Ghasemi H (2010) Myofibroblast stromal presence and distribution in squamous epithelial carcinomas, oral dysplasia and hyperkeratosis. Asian Pac J Cancer Prev 11:359–364Google Scholar
- 28.Kellermann MG, Sobral LM, da Silva SD, Zecchin KG, Graner E, Lopes MA et al (2008) Mutual paracrine effects of oral squamous cell carcinoma cells and normal oral fibroblasts: induction of fibroblast to myofibroblast transdifferentiation and modulation of tumor cell proliferation. Oral Oncol 44:509–517CrossRefGoogle Scholar