Tropical Plant Biology

, Volume 12, Issue 2, pp 98–116 | Cite as

Expression of DREB-Like Genes in Coffea canephora and C. arabica Subjected to Various Types of Abiotic Stress

  • Luana Ferreira Torres
  • Tharyn Reichel
  • Eveline Déchamp
  • Sinara Oliveira de Aquino
  • Karoline Estefani Duarte
  • Gabriel Sergio Costa Alves
  • Anderson Tadeu Silva
  • Michelle Guitton Cotta
  • Tatiana Santos Costa
  • Leandro Eugenio Cardamone Diniz
  • Jean-Christophe Breitler
  • Myriam Collin
  • Luciano Vilela Paiva
  • Alan Carvalho Andrade
  • Hervé Etienne
  • Pierre MarracciniEmail author


The aim of this work was to study the regulation of coffee DREB-like genes in leaves of C. arabica subjected to cold, heat, low relative humidity, exogenous abscisic acid and high light stress, as well as in leaves and roots of drought-tolerant and drought-susceptible clones of Coffea canephora subjected to water limitation. In C. arabica, CaERF017 was the most expressed gene under low temperatures and relative humidity, while low humidity and high temperatures up-regulated the expression of CaERF053 and CaERF014, respectively. Under water limitation, CcDREB1B, CcRAP2.4, CcERF027, CcDREB1D and CcTINY were the most expressed genes mainly in leaves of drought-tolerant C. canephora. On the other hand, expression of the CcERF016, CcRAP2.4 and CcDREB2F genes was highly up-regulated under water limitation in the roots of drought-susceptible C. canephora clone 22. We previously reported fine-tuned regulation of CcDREB1D promoter haplotypes (HP15, HP16 and HP17) in transgenic C. arabica subjected to low humidity. Here, we investigated the regulation of these haplotypes under high light, cold, heat, and abscisic acid (ABA) stress. In apical buds and leaf guard cells, GUS-stained percentages were higher in pHP16L-transformed plants subjected to low humidity, high light and ABA stress than in pHP17L- and pHP15L-transformed plants. We also reported up-regulated expression of the endogenous CaDREB1D gene for both the cold and low humidity in leaves of pHP16L-transformed C. arabica suggesting a key role of this gene in controlling the responses of coffee plants to abiotic stress probably through an ABA-dependent pathway.


Abiotic stress Abscisic acid - coffee - DREB - gene expression - promoter 



This work was carried out under the project of scientific cooperation between Embrapa and Cirad (2011-2017) entitled “Evaluation of coffee (Coffea arabica and C. canephora) germplasm in conditions of water stress for genetic improvement: innovation and recommendations for adaptation of coffee production in dry areas” and under the CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) / COFECUB (Comité Français d’Évaluation de la Coopération Universitaire et Scientifique avec le Brésil) project n°407-2012 (2012-2015) between University of Lavras and Montpellier SupAgro entitled “Genomics and biotechnology applied to the creation of new drought tolerant coffee cultivars”. The authors acknowledge UMR-AGAP – CIRAD, Brazilian Consortium on Coffee R&D, Brazilian Innovation Agency (FINEP), Instituto Nacional de Ciência e Tecnologia do Café/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (INCT/CNPq), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and CAPES/ COFECUB for financial support. We are also grateful to Peter Biggins (CIRAD) for the English revision of the manuscript. This work was carried out under the International Consortium in Advanced Biology (CIBA:

Author’s Contribution

LFT, TR, SOA, KED, TSC, JCB, MGC and TSC extracted RNA samples, performed qPCR experiments and analysed the results. LFT, ED, HE and GSCA carried out genetic transformation, applied abiotic stress on C. arabica and performed GUS staining and microscopy analyses with the help of MC. HE, ACA and PM designed the study, drew up the experimental design and implemented it. LFT, ATS, LECD, LVP, ACA, HE and PM wrote the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of Interest

All authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Luana Ferreira Torres
    • 1
    • 2
  • Tharyn Reichel
    • 1
    • 2
  • Eveline Déchamp
    • 3
  • Sinara Oliveira de Aquino
    • 1
    • 4
  • Karoline Estefani Duarte
    • 1
    • 4
  • Gabriel Sergio Costa Alves
    • 1
    • 4
  • Anderson Tadeu Silva
    • 2
  • Michelle Guitton Cotta
    • 1
    • 4
  • Tatiana Santos Costa
    • 1
    • 4
  • Leandro Eugenio Cardamone Diniz
    • 5
  • Jean-Christophe Breitler
    • 3
  • Myriam Collin
    • 6
  • Luciano Vilela Paiva
    • 1
  • Alan Carvalho Andrade
    • 1
    • 2
    • 4
  • Hervé Etienne
    • 3
  • Pierre Marraccini
    • 4
    • 7
    • 8
    Email author
  1. 1.Universidade Federal de LavrasLavrasBrazil
  2. 2.Embrapa Café, INOVACAFÉLavrasBrazil
  3. 3.UMR IPME, CIRAD, IRDUniversity of MontpellierMontpellierFrance
  4. 4.EMBRAPA Recursos Genéticos e Biotecnologia (LGM)BrasiliaBrazil
  5. 5.EMBRAPA Tabuleiros CosteirosAracajuBrazil
  6. 6.UMR DIADE, CIRAD, IRDUniversity of MontpellierMontpellierFrance
  7. 7.UMR AGAP, CIRAD, IRD, INRAUniversity of MontpellierMontpellierFrance
  8. 8.CIRAD, UMR IPME (Univ. Montpellier, CIRAD, IRD), Agricultural Genetics InstituteLMI RICE2HanoiVietnam

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