Development of InDel Markers for Identification of a Single Mendelian Locus Controlling Leaf Shape in Kenaf (Hibiscus cannabinus)
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Kenaf is one of the most important natural fiber crops. The lack of molecular markers has resulted in a large gap in the improvement of kenaf. Previously, de novo 90,175 unigenes in kenaf were assembled. In this study, 7453 small InDels were called from these uingenes. The indel size <5 bp, 5-10 bp and > 10 bp have frequencies of 71.50%, 23.05%, and 5.45%. 337 InDels locating in genes encoding transcription factors were used to survey polymorphisms with 24 kenaf accessions. Of 337 InDels, 305 (90.5%) successfully amplified at least one fragment and detected polymorphism among the 24 diverse genotypes. Furthermore, one InDel marker, HcID266, was co-segregated with leaf shape, which was inherited as a single Mendelian locus, in the F2 population with 390 individuals. To our knowledge, this is the first large-scale InDels development for kenaf. These InDel markers will be helpful for genetic researches in kenaf.
KeywordsKenaf Hibiscus cannabinus Insertion or deletion Leaf shape Transcription factors
This work was supported by the National Natural Science Foundation of China (31771369), http://www.nsfc.gov.cn/; National Agri-Industry Technology Research System for Crops of Bast and Leaf Fiber, China (nycytx-19-E06), http://184.108.40.206/; Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011); Construction of Germplasm Resources Platform of Bast Fiber Crops in Fujian, China (2010 N2002), http://xmgl.fjkjt.gov.cn/.
Liemei Zhang and Liwu Zhang called the molecular markers. Xuebei Wan, Lilan Zhang and Yi Xu did the DNA extraction and PCR amplification. Xuebei Wan, Jiantang Xu, Lihui Lin, Jianmin Qi and Liwu Zhang prepared for the plant materials and participated in the design of the study. Liemei Zhang and Liwu Zhang critically revised the manuscript. All authors reviewed the manuscript and have given final approval of the version to be published.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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