Development of SSR Markers for Coconut (Cocos nucifera L.) by Selectively Amplified Microsatellite (SAM) and Its Applications
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Coconut is an important tropical fruit and oil crop. Its long generation time, low multiplication rate and recalcitrant seeds make coconut more difficult for breeding and selection. New technologies and extensive resources to evaluate coconut breeding strategies are the demand of today. Molecular markers had the potential to dramatically increase the efficiency and efficacy in the areas of germplasm management, genotype identification and marker-assisted selection of economically important traits of coconut. However, the lack of relevant molecular techniques impedes the development of a new strategy for the genetic improvement of the coconut. In this study, we have successfully developed 84 SSR markers by Selectively Amplified Microsatellite from coconut genome and more than 90% of these SSR showed good transferability to the palm family. The study will enrich the genomic-SSR pool for coconut and also for the other palm tree. Besides, it will provide the scientists with more options for coconut germplasm evaluation, constructing a coconut genetic linkage map and designing the breeding programs for producing superior cultivars of coconut.
KeywordsCoconut Selectively amplified microsatellite (SAM) SSR markers Transferability Palm trees
Researches were supported by the International Science and Technology Cooperation projects of Hainan Province (#KJHZ2014-24) and the Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences (CATAS-No. 1630032012044 and 1630052014002) and “948” projects for Ministry of Agriculture of China (2015-Z25),Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No.1630152017019) and Central Public-interest Scientific Institution Basal Research Fund for Innovative Research Team Program of CATAS (NO. 17CXTD-28).
YW, JL and LZ did the DNA extract and subsequently PCR amplification. YW, YY and YaW participated in the design of the study, performed the data analysis and drafted the manuscript. RQ and AI critically revised the manuscript. HF participated in the design of the study. All authors reviewed the manuscript and have given final approval of the version to be published.
Compliance with Ethical Standards
Conflicts of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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