Genetic structure of Mediterranean fruit fly (Diptera: Tephritidae) populations from Turkey revealed by mitochondrial DNA markers
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Ceratitis capitata is one among the most destructive and economically important agricultural pests worldwide. Despite its economic significance, the population structures of this pest have remained relatively unexplored in the eastern Mediterranean basin. Using two mitochondrial markers, the present study aimed to examining the population genetic structure and diversity of C. capitata populations in Turkey, the region that covers a large part of the eastern Mediterranean area. Our results revealed that the Turkish Mediterranean fruit fly populations are characterized by low levels of genetic diversity and limited population differentiation. For comparison purposes, we merged the sequences identified in the present study with the previously reported sequences from across the world into the data matrix. The haplotype network showed that, unlike the African samples the Mediterranean samples and samples from the new world (America, Pacific region and Australia) did not show any clear pattern of geographical structuring, which indicates that the Mediterranean basin, particularly the eastern Mediterranean region populations, may have played a more important role in the colonization of C. capitata populations to the new world. The results also revealed a close genetic relationship between the Turkish and Iranian populations, suggesting that the Iranian C. capitata populations probably originated from Turkey.
Keywordspopulation structure mitochondrial variation colonizing species medfly Tephritidae Ceratitis capitata
This research was financially supported by the Muğla Sıtkı Koçman University Scientific Research Projects Co-ordination Office (project grant numbers MUBAP 15/075, MUBAP 15/177 and MUBAP 15/171). The authors are grateful to the staff at the Directorate of Plant Protection Research Institutes at corresponding sampling locations for providing samples. The laboratory strain of C. capitata was kindly provided by the Bornova Directorate of Plant Protection Research Institutes (İzmir, Turkey). We also thank the two anonymous reviewers for suggestions which improved the manuscript.
- Aydemir M. (ed.) 2008 Zirai mücadele teknik talimatları cilt V. Agricultural combat technical instructions, volume V, pp. 57–60. Gıda Tarım ve Hayvancılık Bakanlığı, Ankara, Turkey (in Turkish).Google Scholar
- Elekçioğlu N. Z. 2009 Akdeniz Meyve sineği. Biyoloji Bilimleri Araştırma Dergisi 2, 61–65.Google Scholar
- Elfékih S., Makni M. and Haymer D. S. 2010a Detection of novel mitochondrial haplotype variants in populations of the Mediterranean fruit fly, Ceratitis capitata, from Tunisia, Israel and Morocco. J. Appl. Entomol. 134, 647–651.Google Scholar
- Fimiani P. 1989 Mediterranean region. In Fruit flies: their biology, natural enemies and control (ed. A. S. Robinson and G. H. Hooper), vol. 3A, pp. 39–50. Elsevier, Amsterdam.Google Scholar
- Gallo D. N. O., Wiendel F. M., Silvera Neto S. and Ricardo P. L. C. 1970 Manual de entomologia agronomica. Ceres, Sao Paulo.Google Scholar
- Gallo-Franco J. G., Velasco-Cuervo S. M., Aguirre-Ramirez E., Obando R. G., Carrejo N. S. and Toro-Perea N. 2017 Genetic diversity and population structure of Anastrepha striata (Diptera: Tephritidae) in three natural regions of southwestern Colombia using mitochondrial sequences. Genetica 145, 79–89.CrossRefGoogle Scholar
- Hooper G. H. S. and Drew R. A. I. 1989 Australia and South Pacific islands. In Fruit flies: their biology, natural enemies and control (ed. A. S. Robinson and G. H. Hooper) vol. 3A, pp. 67–72. Elsevier, Amsterdam.Google Scholar
- Mantel N. 1967 The detection of disease clustering and a generalized regression approach. Cancer Res. 27, 209–220.Google Scholar
- Ochando M. D., Reyes A., Callejas C., Segura D. and Fernández P. 2003 Molecular genetic methodologies applied to the study of fly pests. Trends Entomol. 3, 73–85.Google Scholar
- Reyes A. and Ochando M. D. 1997 Fitness of mitochondrial DNA haplotypes in Ceratitis capitata. IOBC/wprs Bull. 20, 175–185.Google Scholar