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Journal of Genetics

, 98:41 | Cite as

Identification and expression profiling of MSY genes of yak for bull fertility

  • Partha Pratim Das
  • G. Krishnan
  • Juwar Doley
  • Tapas Kumar Biswas
  • Vijay Paul
  • Prithiviraj Chakravarty
  • Sitangshu Mohan Deb
  • Pranab Jyoti DasEmail author
Research Article
  • 46 Downloads

Abstract

Yak (Bos grunniens) is a unique bovine species and considered as lifeline of highlanders. The male subfertility in yak is a matter of concern that causes huge economic loses. The spermatogenesis and male reproduction machinery are critically governed by Y-linked genes which tend to acquire necessary information in the course of evolution. The Y-linked fertility genes are present in multiple copies with testis-limited expression. To understand this novel complexity, 12 male-specific region of Y chromosome (MSY) genes have been studied in the yak. Targeted genes are amplified in male and female genomic DNA and confirmed the male-derived specificity. Moreover, testis and sperm-specific expressions of MSY genes are distinct among different tissues. The quantitative polymerase chain reaction results validate the expression pattern of these genes in various tissues with predominant expression in testis and sperm. The sequencing of resultant yak MSY genes gives significant result and shows similarity with cattle (Bos indicus), but few nucleotide mismatches define the proposition of infertile male in the \(\hbox {F}_{{1}}\) hybrid of cattle and yak. The identified MSY genes can be used to establish male-specific characteristics and to differentiate male and female yak genotypically. Further, these genes may act as valuable resources to understand the capacity of spermatogenesis, embryogenesis, cellular growth, azoospermia and male subfertility in the yak.

Keywords

yak Y-chromosome male-specific region of Y chromosome gene male-fertility testis spermatozoa 

Notes

Acknowledgements

The authors are thankful to the Director, ICAR-NRCY for granting permission to avail the facilities to carry out this research project. The study was supported by ICAR-NRC on Yak Institutional project grant 2013–2015.

Supplementary material

12041_2019_1091_MOESM1_ESM.docx (2.8 mb)
Supplementary material 1 (docx 2847 KB)

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Partha Pratim Das
    • 1
  • G. Krishnan
    • 2
  • Juwar Doley
    • 1
  • Tapas Kumar Biswas
    • 3
  • Vijay Paul
    • 1
  • Prithiviraj Chakravarty
    • 1
  • Sitangshu Mohan Deb
    • 4
  • Pranab Jyoti Das
    • 1
    • 5
    Email author
  1. 1.Indian Council of Agricultural Research-National Research Centre on YakWest KamengIndia
  2. 2.Indian Council of Agricultural Research-National Institute of Animal Nutrition and PhysiologyBengaluruIndia
  3. 3.Indian Council of Agricultural Research-Indian Veterinary Research Institute Eastern Regional StationKolkataIndia
  4. 4.Indian Council of Agricultural Research-National Dairy Research InstituteKarnalIndia
  5. 5.Indian Council of Agricultural Research-National Research Centre on PigRani, GuwahatiIndia

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