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Journal of Genetics

, 98:38 | Cite as

Identification of novel polymorphism in buffalo stanniocalcin-1 gene and its expression analysis in mammary gland under different stages of lactation

  • S. K. Mishra
  • P. K. Dubey
  • S. Goyal
  • S. Singh
  • S. K. Niranjan
  • V. Vohra
  • M. Mukesh
  • R. S. KatariaEmail author
Research Note
  • 10 Downloads

Abstract

Stanniocalcin-1 (STC1) is secreted by the variety of tissues having a major role in the regulation of calcium ions in the involuting mammary gland. The present work aims to sequence and structural characterization as well as expression profiling of STC1 gene in buffalo. Polymorphism identified in the \(3^{\prime }\)-untranslated region (UTR) was analysed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyping in riverine and swamp buffaloes. Expression profiling of STC1 was performed in different lactation stages of mammary gland and peripheral blood mononuclear cells to study the impact of \(3^{\prime }\)-UTR polymorphism on its expression. Different polymorphic sites were detected in the entire coding and noncoding regions of riverine and swamp buffaloes, including two INDELs. An identified polymorphic nucleotide locus A324G, having target sites for two miRNAs, namely bta-miR-2382 and bta-miR-1343, reported in cattle, was genotyped by PCR-RFLP to reveal variable allelic distribution among swamp and riverine buffaloes. Gene expression profiling across buffalo mammary tissues representing different lactation stages showed maximum expression of the STC1 gene in the involuting mammary gland. Ruminants’ specific genetic variation has been observed in STC1 and its implication in buffalo mammary gland involution as well as coregulation of gene expression through miRNA binding in the \(3^{\prime }\)-UTR is suggested.

Keywords

STC1 gene polymorphism \(3^{\prime }\)-untranslated region micro-RNA target sites expression analysis Bubalus bubalis 

Notes

Acknowledgements

The authors wish to thank the Director, National Bureau of Animal Genetic Resources, Indian Council of Agricultural Research, for providing necessary funding to carry out the work.

Supplementary material

12041_2019_1082_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (doc 1547 KB)

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • S. K. Mishra
    • 1
  • P. K. Dubey
    • 2
  • S. Goyal
    • 1
  • S. Singh
    • 1
  • S. K. Niranjan
    • 1
  • V. Vohra
    • 1
  • M. Mukesh
    • 1
  • R. S. Kataria
    • 1
    Email author
  1. 1.ICAR-National Bureau of Animal Genetic ResourcesKarnalIndia
  2. 2.Lewis Ketz School of MedicineTemple UniversityPhiladelphiaUSA

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