Identification of sex using SBNO1 gene in the Chinese softshell turtle, Pelodiscus sinensis (Trionychidae)
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The Chinese softshell turtle exhibits ZZ/ZW sex determination. To identify the sex of embryos, juvenile and adult individuals, we designed two pairs of polymerase chain reaction primers, SB1-196, which amplifies a fragment of 196 bp in the female and the other, CK1-482, which amplifies the 482-bp fragment in both the sexes. It is validated in 24 adult turtles of known sex, sampled from three different locations. This one-step sexing technique is rapid and easy to perform and is reported for the first time.
Keywordspolymerase chain reaction sex identification sex chromosome molecular sexing reptile Chinese softshell turtle
This work was supported by the National Key Programme (2016YFC0503200) from the Ministry of Science and Technology of China, a special grant from the State Forestry Administration, and the Fundamental Research Funds for the Central Universities of China. We are grateful to Xiaoshan Tianfu Bio-Technology, Hangzhou, Zhejiang Province, China, for providing samples of the Chinese softshell turtles of Japanese population.
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