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Journal of Chemical Sciences

, 130:132 | Cite as

Modification of fatty acid vesicle using an imidazolium-based surface active ionic liquid: a detailed study on its modified properties using spectroscopy and microscopy techniques\(^{\S }\)

  • Shreya Roy
  • Sarthak Mandal
  • Pavel Banerjee
  • Nilmoni Sarkar
Regular Article
  • 110 Downloads

Abstract

Fatty acid vesicles have attracted views as model protocell membranes in understanding the emergence of life, but their properties can be further modified in the presence of some external molecules. In this work, we have investigated the spontaneous formation of large unilamellar vesicles (LUVs) of oleic acid in aqueous medium in presence of a popular imidazolium-based cationic surface active ionic liquid (SAIL) \([\hbox {C}_{16}\hbox {mim}]\hbox {Cl}\) and studied the micelle–vesicle transition of aqueous \([\hbox {C}_{16}\hbox {mim}]\hbox {Cl}\) solution in presence of different molar fractions (f) of oleic acid. This newly formed oleic \(\hbox {acid}/[\hbox {C}_{16}\hbox {mim}]\hbox {Cl}\) vesicles exhibit some modified properties compared to the pure fatty acid vesicles. Unlike pure fatty acid vesicles, these vesicles are stable in the pH range of 2 to 11.2. We have observed the fusion process of these oleic acid/SAIL vesicles to form giant unilamellar vesicles (GUVs) in presence of low concentration of NaCl solution. To investigate the dynamics of different oleic \(\hbox {acid}/[\hbox {C}_{16}\hbox {mim}]\hbox {Cl}\) self-assemblies, we have used fluorescence correlation spectroscopy (FCS). The translational diffusion behavior of three different dyes, Rhodamine 6G, DCM and Pyrromethene 597, which are non-covalently bound to the different regions of the oleic acid/SAIL self-assemblies, have been determined using FCS during the micelle–vesicle transition and upon varying the pH of the vesicular solution.

Graphical Abstract

Oleic acid vesicles prepared in presence of a surface active ionic liquid \([\hbox {C}_{16}\hbox {mim}]\hbox {Cl}\) have been characterized. Modified properties of these vesicles like stability towards a greater pH range as compared to pure oleic acid vesicles and fusion of vesicles in presence of low concentration of NaCl solution have been studied.

Keywords

Model membrane micelle–vesicle transition single molecule spectroscopy ionic liquid fluorescence correlation spectroscopy fluorescence lifetime imaging microscopy 

Notes

Acknowledgements

N.S. gratefully acknowledges SERB, Department of Science and Technology (DST), Government of India for providing a generous research grant. S.R., S.M. and P. B. are thankful to UGC and CSIR for research fellowships. We are thankful to Dr. Niloy Kundu and Dr. Arpita Roy for helpful discussions.

Supplementary material

12039_2018_1532_MOESM1_ESM.pdf (877 kb)
Supplementary material 1 (pdf 876 KB)

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Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  1. 1.Department of ChemistryIndian Institute of TechnologyKharagpurIndia
  2. 2.Centre for Innovations in MedicineArizona State UniversityTempeUnited States

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