Biophysical methods for quality evaluation of decellularized and recellularized tissue-engineered constructs of organs and tissues
Tissue engineering is rapidly growing now and can become a promising alternative to transplantation of organs and tissues, as it is devoid of major shortcomings of transplantology, such as acute shortage, complexity of selection, delivery and storage of donor material, lifelong immunosuppressive therapy. One of the most widely known methods of obtaining biological scaffolds for the subsequent creation of tissue-engineered constructs of organs and tissues is decellularization. The evaluation of the quality of the obtained scaffolds, based on the study of the viability of cell structures in decellularized and recellularized matrices, is one of the priorities of modern regenerative medicine worldwide. In this investigation, the biophysical criteria of decellularization and recellularization of tissue-engineered constructs based on the evaluation of the generation of free radicals in native, decellularized and recellularized tissues by EPR spectroscopy and chemoluminescence in a complex assessment of the quality of biological matrixes obtained are considered using intrathoracic organs and tissues of rats. It has been established that the intensity indices of free radical generation in native and recellularized tissues of animal organs, as well as in decellularized matrices, can serve as one of the express criteria for quantitative assessment of cell structures viability.
KeywordsChemoluminescence decellularization EPR spectroscopy recellularization tissue-engineered constructs
The investigation was carried out under the support of the complex research project ‘Cell mechanisms of intrathoracic organs and tissues regeneration. Development of tissue-engineered constructs using biological and synthetic scaffolds’, and under the support of the state assignment of the Ministry of Education and Science of the Russian Federation, project No. 6.5882.2017.
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