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Journal of Biosciences

, 44:105 | Cite as

Biosensor design using an electroactive label-based aptamer to detect bisphenol A in serum samples

  • Maryam Nazari
  • Soheila KashanianEmail author
  • Ronak Rafipour
  • Kobra Omidfar
Article
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Abstract

A new and simple procedure was applied to detect bisphenol A (BPA) based on a BPA aptamer and its complementary strand (Comp. Str.). An electrode was modified with a mixture of carboxylated multiwalled carbon nanotubes and chitosan. The Comp. Str. was immobilized on a modified-glassy carbon electrode (GCE) surface via covalent binding. After the incubation of the aptamer with the electrode surface, it could interact with the Comp. Str. In the presence of BPA, its aptamer will interact with the analyte, resulting in some changes in the configuration and leading to separation from the electrode surface. Due to the attached ferrocene (Fc) group on the 5′ head of the aptamer, the redox current of Fc has reduced. This aptasensor can sense the level of BPA in the linear range of 0.2–2 nM, with a limit of detection of 0.38 nM and a sensitivity of 24.51 μA/nM. The proposed aptasensor showed great reliability and selectivity. The acceptable selectivity is due to the specificity of BPA binding to its aptamer. The serum sample was used as a real sample; the aptasensor was able to effectively recover the spiked BPA amounts. It can on-site monitor the BPA in serum samples with acceptable recoveries.

Keywords

Aptasensor bisphenol A chitosan ferrocene multiwalled carbon nanotubes 

Notes

References

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Maryam Nazari
    • 1
    • 2
  • Soheila Kashanian
    • 3
    • 4
    Email author
  • Ronak Rafipour
    • 5
  • Kobra Omidfar
    • 1
    • 6
  1. 1.Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences InstituteTehran University of Medical SciencesTehranIran
  2. 2.Faculty of ChemistryRazi UniversityKermanshahIran
  3. 3.Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC)Razi UniversityKermanshahIran
  4. 4.Nano Drug Delivery Research CenterKermanshah University of Medical ScienceKermanshahIran
  5. 5.Department of Chemistry, Kermanshah BranchIslamic Azad UniversityKermanshahIran
  6. 6.Endocrine and Metabolism Research Center, Endocrinology and Metabolism Research InstituteTehran University of Medical SciencesTehranIran

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