Import of human miRNA-RISC complex into Plasmodium falciparum and regulation of the parasite gene expression

  • Vishal Dandewad
  • Arya Vindu
  • Jomon Joseph
  • Vasudevan SeshadriEmail author
Brief communication


During its life cycle, the malarial parasite Plasmodium goes through different asexual stages in human blood, and asexual and sexual stage in mosquito. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In case of Plasmodium, due to relative paucity of the transcription factors, it is postulated that post-transcriptional regulation plays an important role in stage-specific gene expression. Although miRNA-mediated gene regulation has been well-established to function in post-transcriptional regulation in many eukaryotes, existence of such a phenomenon or the presence of miRNA-associated factors in Plasmodium remains unclear. A number of miRNAs are shown to be imported into Plasmodium falciparum from erythrocytes and role of these miRNAs is not understood. Here we show that human Argonaute 2 (hAgo2) a component of the miRISC complex is imported by P. falciparum. In the parasite hAgo2 exists as in a complex with specific human miRNAs like let-7a and miR15a which can potentially target the Plasmodium genes Rad54 and Lipid/sterol:H+ symporter respectively. We show that hAgo2 associates with Rad54, Lipid/sterol:H+ symporter and other P. falciparum transcripts. These results highlight the existence of a mechanism by which malarial parasite imports hAgo2-miRNA complex from the host cells to regulate its gene expression.


Argonaute miRNA Plasmodium falciparum 



We thank Dr. Swati Patankar for the gift of 3D7 culture. This work has been funded by the grant to VS from NCCS (intramural) and Department of Science and Technology, Government of India (EMR/2014/001093). VD was supported by fellowship from Department of Biotechnology, India and AV was supported fellowship from Council for Scientific and Industrial Research, India.

Supplementary material

12038_2019_9870_MOESM1_ESM.pdf (637 kb)
Supplementary material 1 (PDF 637 kb)


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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.National Centre for Cell SciencePuneIndia
  2. 2.Department of BiotechnologySavitribai Phule Pune UniversityPuneIndia

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