Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases

  • Shou-Feng Zhao
  • Zhe Chi
  • Guang-Lei Liu
  • Zhong Hu
  • Long-Fei Wu
  • Zhen-Ming ChiEmail author


Pyruvate carboxylase (Pyc) catalyzes formation of oxaloacetic acid from pyruvic acid by fixing one mole of CO2. Many evidences have confirmed that biosynthesis of some different kinds of organic acids and intracellular and extracellular lipids is driven by Pyc and over-expression of the PYC gene in the industrial microorganisms can promote production of the different kinds of organic acids and intracellular and extracellular lipids. Therefore, the Pyc from different sources is regarded as a key enzyme in microbial biotechnology and is an important target for metabolic engineering of the industrial microbial strains. However, very little is known about the native Pycs and their functions and regulation in the industrial microorganisms.


C4 dicarboxylic acids industrial microorganisms lipids metabolic engineering pyruvate carboxylase 



This research was supported by the National Natural Science Foundation of China (Grant No. 31770061).


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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Shou-Feng Zhao
    • 1
    • 2
  • Zhe Chi
    • 1
    • 3
  • Guang-Lei Liu
    • 1
    • 3
  • Zhong Hu
    • 4
  • Long-Fei Wu
    • 1
  • Zhen-Ming Chi
    • 1
    Email author
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoPeople’s Republic of China
  2. 2.Central LaboratoriesQingdao Municipal HospitalQingdaoPeople’s Republic of China
  3. 3.Key Laboratory of Marine Genetics and BreedingOcean University of ChinaQingdaoPeople’s Republic of China
  4. 4.Department of BiologyShantou UniversityShantouPeople’s Republic of China

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