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Regeneration and evaluation of somaclones of sugarcane variety Co86032 for yellow leaf disease resistance and yield traits

  • Praveen KonaEmail author
  • M Hemanth Kumar
  • K H P Reddy
  • T M Hemalatha
  • D M Reddy
  • N P Eswar Reddy
  • P Latha
Article
  • 7 Downloads

Abstract

The present investigation was focussed on regeneration, evaluation and screening of somaclones for yellow leaf disease (YLD) resistance using in vitro mutagenesis from a popular susceptible sugarcane variety Co86032 using four chemical mutagens at three levels of concentration (sodium azide (SA) at 0.5 mg L−1, 1.0 mg L−1, 1.5 mg L−1; sodium nitrite (SN) at 3 mg L−1, 5 mg L−1, 7 mg L−1; ethyl methane sulphonate (EMS) at 0.6 μM L−1, 0.8 μM L−1, 1.0 μM L−1 and 2,4 D at 4 mg L−1, 5 mg L−1, 6 mg L−1). A total of 1138 tissue culture seedlings obtained were evaluated for virus resistance both in natural field conditions and in controlled greenhouse condition after aphid vector transmission and presence or absence of virus was observed by visual screening and reverse transcription-polymerase chain reaction method. Four out of 207 asymptomatic plants (16T22, 16T23, 16T29 and 16T31) were devoid of virus coat protein band and were considered to be YLD resistant. The obtained resistance somaclones showed inferior yield traits so they have to be exploited as parents in hybridization programmes with commercial varieties to impart YLD resistance ultimately yielding agronomically superior YLD-resistant varieties in sugarcane.

Keywords.

RT-PCR somaclones sugarcane YLD 

Notes

Acknowledgements

The authors acknowledge Agricultural Research Station, Perumallapalli, staff for providing the lab facilities to carry out the research programme. They also acknowledge Acharya N.G. Ranga Agricultural University, Andhra Pradesh.

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.Crop Improvement UnitICAR-DGRJunagadhIndia
  2. 2.Agricultural Research StationPerumallapalleIndia
  3. 3.Department of Genetics and Plant BreedingS.V. Agricultural CollegeTirupatiIndia
  4. 4.Institute of Frontier Technologies, RARSTirupatiIndia

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