Journal of Biosciences

, 44:20 | Cite as

Comparative physiological and leaf proteome analysis between drought-tolerant chickpea Cicer reticulatum and drought-sensitive chickpea C. arietinum

  • Sertan ÇevikEmail author
  • Gürler Akpinar
  • Aytunç Yildizli
  • Murat Kasap
  • Kübra Karaosmanoğlu
  • Serpil Ünyayar


Comparative physiological and proteomic analysis were performed to understand the stress responses of two chickpea species (C. reticulatum and C. arietinum) against drought. Our study revealed that drought stress reduced root length, leaf water content, and enhanced free proline content in both species. Effect of drought stress appeared to be greater in C. arietinum compared to C. reticulatum. A total of 24 differently expressed proteins were identified by using MALDI-TOF/TOF-MS/MS in response to drought. The proteins involved in photosynthesis and energy mechanisms were up-regulated in C. reticulatum and down-regulated in C. arietinum under drought. Our results suggest that the photosynthesis capacity of C. reticulatum is greater than that of C. arietinum under drought stress. Abundance of proline and sucrose biosynthesis related proteins, glutamine synthetase and cyctosolic fructose-bisphosphate aldolase, respectively, also increased in C. reticulatum under drought stress. The findings of this proteome analysis will help in understanding the mechanism of drought resistance in chickpea and may be also helpful in developing drought-resistant transgenic plants.


Chickpea drought stress physiological analysis proteomics 



ascorbate peroxidase


cytochrome c oxidase


fructose-bisphosphate aldolase


ferredoxin-NADP reductase




glutamine synthetase


glyceraldehyde-3-phosphate dehydrogenase


ısoflavone reductase


light-harvesting chlorophyll a/b-binding protein


leaf water potential


oxygen evolving enhancer protein 1


oxygen evolving enhancer protein 2


phosphoglycerate kinase


reactive oxygen species


relative water content




two-dimensional polyacrylamide gel electrophoresis



The authors would like to thank Prof. Dr. Cengiz Toker (Department of Field Crops, Faculty of Agriculture, Akdeniz University, Antalya, Turkey) for providing the plant material. The manuscript was linguistically supported by the Technology Transfer Office Academic Writing Center of Mersin University. This work was supported by the University of Mersin; Project number is BAP-FBE BB (SÇ) 2012-4 DR.

Supplementary material

12038_2018_9836_MOESM1_ESM.docx (737 kb)
Supplementary material 1 (DOCX 737 kb)
12038_2018_9836_MOESM2_ESM.xlsx (32 kb)
Supplementary material 2 (XLSX 31 kb)


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© Indian Academy of Sciences 2019

Authors and Affiliations

  1. 1.Vocational School of MutMersin UniversityMersinTurkey
  2. 2.Medical Biology Department/Dekart Proteomics LaboratoryKocaeli UniversityKocaeliTurkey
  3. 3.Biology Department, Art and Science FacultyMersin UniversityMersinTurkey
  4. 4.Biomedical Engineering Department, Technology FacultyKocaeliTurkey

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