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Journal of Biosciences

, 44:10 | Cite as

Measles virus phosphoprotein inhibits apoptosis and enhances clonogenic and migratory properties in HeLa cells

  • Sankhajit Bhattacharjee
  • Rishi Kumar Jaiswal
  • Pramod Kumar YadavaEmail author
Article
  • 112 Downloads

Abstract

Measles virus is the causative agent of measles, a major cause of child mortality in developing countries. Two major proteins, coded by the viral genome, are nucleocapsid protein (N) and phosphoprotein (P). The N protein protects the viral genomic RNA and forms ribonucleoprotein complex (RNP) together with P protein. MeV-P protein recruits the large protein (L), i.e. viral RNA-depended RNA polymerase (RdRp), to ensure viral replication in host cell. Apoptogenic properties of N protein of Edmonston vaccine strain have been established in our lab previously. We investigated the role of MeV-P protein of Edmonston vaccine strain as modulator of apoptosis in cervical cancer cell line (HeLa) and found that MeV-P protein is anti-apoptotic and enhances cell proliferation. Measles virus is considered to be innately oncotropic virus. However, the anti-apoptotic property of MeV-P protein raises important concerns while adopting this virus as an anti-cancer therapeutic tool.

Keywords

Anti-apoptosis cell migration clonogenicity, HeLa cells measles virus phosphoprotein Vimentin 

Abbreviations

MeV

measles virus

MeV-P

measles virus phosphopreotein

MeV-N

measles virus nucleocapsid protein

RNP

ribonucleoprotein

PNT

N-terminal domain of MeV-P

PCT

C-terminal domain of MeV-P

ROS

reactive oxygen species

PLAU(uPA)

urokinase like plasminogen activator

Notes

Acknowledgements

We are thankful to Prof Martin Billeter for providing the plasmids. We thank Mrs Sarika Gupta for expert assistance with the flow cytometry experiments. Our study was supported by grants, which were received from UGC and DST, India. SB is the recipient of a Senior Research Fellowship of UGC.

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Copyright information

© Indian Academy of Sciences 2019

Authors and Affiliations

  • Sankhajit Bhattacharjee
    • 1
  • Rishi Kumar Jaiswal
    • 1
  • Pramod Kumar Yadava
    • 1
    Email author
  1. 1.Applied Molecular Biology Laboratory, School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia

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